| 基于连接酶检测反应的PRL基因多态与清远麻鸡繁殖性能的相关性研究 |
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| 引用本文： | 束婧婷,李慧芳,邹剑敏,张莹,陈宽维,尹平安,韩威,宋卫涛.基于连接酶检测反应的PRL基因多态与清远麻鸡繁殖性能的相关性研究[J].畜牧兽医学报,2011,42(8). |
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| 作者姓名： | 束婧婷 李慧芳 邹剑敏 张莹 陈宽维 尹平安 韩威 宋卫涛 |
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| 作者单位： | 1. 中国农业科学院家禽研究所,扬州,225125
2. 广东天农食品有限公司,清远,511827 |
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| 基金项目： | 现代农业产业技术体系建设专项资金; 国家高技术研究发展计划(863计划)(2011AA100301); 江苏省自然科学基金(BK2009177); 江苏省家禽科学研究所青年(博士)基金(JQ2009001) |
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| 摘 要： | 旨在建立一个基于聚合酶链反应-连接酶检测反应的基因多态性并行检测系统,探讨鸡催乳素(PRL)基因的多态性,并分析其对优良地方鸡种——清远麻鸡繁殖性能的遗传效应。本研究应用PCR-LDR方法检测PRL8052T/C和PRL8113G/C基因型,并使用SAS软件进行最小二乘分析。结果,在8052T/C位点上,CT基因型个体的52周产蛋数显著高于TT型个体;在8113G/C位点上,CG基因型个体的开产日龄显著低于GG型个体,而其52周龄产蛋数却显著高于GG型个体。2个位点的单倍型对开产日龄和52周产蛋数也存在显著的效应,CTCG型个体具有较早的开产日龄和最高的52周龄产蛋数。结果表明,PRL基因多态性与清远麻鸡繁殖性状有相关关系,PRL 8052T/C和PRL 8113G/C杂合基因型可能是提高鸡繁殖性能的分子标记,利用单倍型进行标记辅助选择可能会获得更大的遗传进展。
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| 关 键 词： | 连接酶检测反应 PRL基因 繁殖性状 基因多态性 清远麻鸡 |
A Study on the Relativity between PRL Gene Polymorphisms and Reproductive Traits of Qingyuan Partridge Chicken Based on Ligase Detection Reaction |
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| SHU Jing-ting,LI Hui-fang,ZOU Jian-min,ZHANG Ying,CHEN Kuan-wei,YIN Ping-an,HAN Wei,SONG Wei-tao.A Study on the Relativity between PRL Gene Polymorphisms and Reproductive Traits of Qingyuan Partridge Chicken Based on Ligase Detection Reaction[J].Acta Veterinaria et Zootechnica Sinica,2011,42(8). |
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| Authors: | SHU Jing-ting LI Hui-fang ZOU Jian-min ZHANG Ying CHEN Kuan-wei YIN Ping-an HAN Wei SONG Wei-tao |
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| Institution: | SHU Jing-ting1,LI Hui-fang1,ZOU Jian-min1,ZHANG Ying2,CHEN Kuan-wei1,YIN Ping-an2,HAN Wei1,SONG Wei-tao1 (1.Poultry Institute,Chinese Academy of Agricultural Sciences,Yangzhou 225125,China,2.Guangdong Tiannong Food Ltd.,Qingyuan 511827,China) |
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| Abstract: | To establish a parallel typing system based on ligase detection reaction,and to detect the polymorphisms of PRL gene as well as its genetic effect on age at first laying,egg weight at first laying,body weight at first laying,average egg weight at 40 weeks of age,average egg weight at 50 weeks of age and total egg number at 52 weeks of age in Qingyuan Partridge chicken.PCR-LDR technique was used to identify the genotypes of PRL 8052T/C and PRL 8113G/C,and the data were analyzed by the least square analysis u... |
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| Keywords: | ligase detection reaction PRL gene reproductive traits gene polymorphisms Qingyuan Partridge chicken |
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