| 枯草芽孢杆菌拮抗病原菌对细胞微丝的影响 |
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| 引用本文： | 叶小兰,李鹏成,杨倩.枯草芽孢杆菌拮抗病原菌对细胞微丝的影响[J].畜牧兽医学报,2011,42(8). |
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| 作者姓名： | 叶小兰 李鹏成 杨倩 |
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| 作者单位： | 南京农业大学动物医学院,南京,210095 |
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| 基金项目： | 国家自然科学基金项目(30871858); 江苏省支撑计划(BE200830155) |
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| 摘 要： | 本研究旨在探讨枯草芽孢杆菌拮抗2种病原菌(鼠伤寒沙门氏菌和产肠毒素大肠杆菌)对人结肠癌细胞Caco-2细胞中微丝的影响。将枯草芽孢杆菌及上述2种病原菌加到Caco-2细胞上,用异硫氰酸荧光素-鬼笔环肽标记细胞微丝,在倒置荧光显微镜下观察细胞微丝的变化;利用Western blotting检测细胞微丝调节蛋白Rac1蛋白的表达变化。结果显示正常Caco-2细胞内微丝呈散在的平行排列;加鼠伤寒沙门氏菌后微丝呈小区域聚集;加产肠毒素大肠杆菌后细胞微丝重排聚集在细胞中央放射状分布或分布紊乱;单独加枯草芽孢杆菌后细胞微丝排列方式变化不大;同时分别加2种病原菌和枯草芽孢杆菌后,大部分的细胞内微丝排列整齐,只是在少部分细胞内存在微丝的聚集。产肠毒素大肠杆菌、鼠伤寒沙门氏菌可使细胞微丝调节蛋白Rac1的表达显著上调,而枯草芽孢杆菌则可抑制病原菌对Rac1的影响。本试验证明产肠毒素大肠杆菌、鼠伤寒沙门氏菌可影响细胞微丝的表达和分布;而枯草芽孢杆菌及其培养上清液可抑制病原菌的这种破坏作用。
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| 关 键 词： | 病原菌 枯草芽孢杆菌 Caco-2细胞 微丝 |
Effects of Bacillus subtilis Against Pathogens on Actin Filament |
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| YE Xiao-lan,LI Peng-cheng,YANG Qian.Effects of Bacillus subtilis Against Pathogens on Actin Filament[J].Acta Veterinaria et Zootechnica Sinica,2011,42(8). |
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| Authors: | YE Xiao-lan LI Peng-cheng YANG Qian |
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| Institution: | YE Xiao-lan,LI Peng-cheng,YANG Qian (College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | The aim of this study was to investigate the effect of Bacillus subtilis against pathogens(Salmonella typhimurium and enterotoxic Escherichia coli) on the actin filament of Caco-2 cell.Bacillus subtilis and the pathogens were added to Caco-2 cell,the actin filaments in the cells was dyed by FTTC-phalloidin and the changes of actin filaments was examined by inverted fluorescence microscope.The regulatory protein Rac1 of actin filaments was measured by Western Blotting.The results showed that the actin filame... |
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| Keywords: | pathogenic bacteria Bacillus subtilis Caco-2 cell actin filament |
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