Cryopreservation of Black Bengal buck semen: Effects of diluents and freezing on sperm motility and morphology |
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| Authors: | Sadia AFROZ Md Rashedul ISLAM MAM Yahia KHANDOKER Quzi Sharmin AKTER |
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| Institution: | Department of Animal Breeding and Genetics, Bangladesh Agricultural University (BAU), Mymensingh, and;National Institute of Biotechnology (NIB), Atomic Energy Research Establishment (AERE), EPZ, Savar, Dhaka, Bangladesh |
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| Abstract: | Semen from Black Bengal bucks was collected to establish a cooling protocol (to ?196°C) for buck semen preservation, and to study the effect of freezing on sperm motility and morphology. Semen was diluted with diluents (Triladyl & Tris) and cryoprotectants, filled into straws, sealed, cooled (to 5°C) and equilibrated. After dilution, motility ranged from 75.00% to 76.67% and from 73.33% to 80.00% in Triladyl and Tris diluents, respectively. Motility of sperm after cooling to 5°C in Triladyl and Tris diluents ranged from 65.00% to 66.67% and from 63.33% to 70.00%, respectively. After equilibration in straws, the semen was subjected to a freezing protocol in a computer-controlled biofreezer CL-3000 (cooling at 10°C per minute, from 5°C to ?80°C) and plunged into liquid nitrogen. Sperm motility of re-thawed semen varied from 38.33% to 43.33% and from 6.00% to?6.67% in Triladyl and Tris diluents, respectively. Sperm morphology of re-thawed semen was studied and head damage or cryoinjury was found in 2–3% of sperm in Triladyl diluents and 3–6% in Tris diluents. Whether the differences of sperm motility and head damage reflect fertility after artificial insemination is yet unknown and needs to be studied further. |
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| Keywords: | Black Bengal buck semen cryoinjury cryopreservation Triladyl Tris |
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