|
|||||
|
|
Development of an ISSR-derived PCR marker linked to nematode resistance (Ha2) in spring barley |
|
| Authors: | C Dayteg M Rasmussen S Tuvesson A Merker and A Jahoor |
| Institution: | SW Seed, SE-26881 Svalöv, Sweden, E-mail:;;Department of Crop Science, Swedish University of Agricultural Sciences, Box 44, SE-23053 Alnarp, Sweden;;Plant and Soil Science Laboratory, Department of Agricultural Sciences, The Royal Veterinary and Agriculture University, Thorvaldssensvej 40, DK-1871 Fredriksberg, C, Copenhagen, Denmark |
| Abstract: | Cereal cyst nematodes ( Heterodera avenae Woll.) are economically damaging barley parasites in most cereal growing areas of the world and the development of resistant cultivars is the best measure against the pathogen. An ISSR (inter-simple sequence repeat) marker identified as closely linked with the H. avenae race 1 and 2 resistance gene ( Ha2 ) has been converted into a codominant sequence characterized amplified region marker ( Ha2S18 ) and mapped in barley 'SW Buddy' × 'SW Cecilia' DH population at 4.3 cM from the Ha2 locus on the long arm of chromosome 2H. The potential usefulness of Ha2S18 in large scale marker assisted selection schemes has been evaluated in a broad genetic background and is an important complement to the bioassay and to other linked DNA-markers for this trait. |
| Keywords: | Hordeum vulgare Heterodera avenae Ha2 ISSR SCAR cereal cyst nematode resistance |