| 黑番鸭GH基因的克隆与序列分析 |
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| 引用本文： | 朱志明,黄种彬,陈晖,钟志新,郑嫩珠,缪中纬.黑番鸭GH基因的克隆与序列分析[J].福建畜牧兽医,2011,33(5):16-18. |
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| 作者姓名： | 朱志明 黄种彬 陈晖 钟志新 郑嫩珠 缪中纬 |
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| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福州,350013
2. 福建省石狮市水禽保种中心,福建石狮,362700 |
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| 基金项目： | 福建省科技重点项目，福建省公益类科研院所专项，国家现代农业产业技术体系建设专项 |
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| 摘 要： | 根据GenBank中公布的鸭GH基因序列,设计4对引物,以黑番鸭血液基因组DNA为模板,采用PCR方法,扩增出GH基因4650bp的DNA序列,该序列包含了完整的内含子和外显子序列,由5个外显子和4个内含子组成。进一步将克隆的黑番鸭GH基因编码的氨基酸序列与GenBank中其他物种的该基因氨基酸序列进行同源性分析和聚类...
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| 关 键 词： | 黑番鸭 GH基因 克隆 序列分析 |
Cloning and Sequence analysis of GH gene in Black Muscovy Duck |
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| Zhu Zhiming Huang Zhongbin Chen Hui Zhong Zhixin Zheng Nenzhu Miao Zhongwei.Cloning and Sequence analysis of GH gene in Black Muscovy Duck[J].Fujian Journal of Animal Husbandry and Veterinary,2011,33(5):16-18. |
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| Authors: | Zhu Zhiming Huang Zhongbin Chen Hui Zhong Zhixin Zheng Nenzhu Miao Zhongwei |
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| Institution: | Zhu Zhiming Huang Zhongbin Chen Hui Zhong Zhixin Zheng Nenzhu Miao Zhongwei(1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013;2.ShiShi Waterfowl Conservation Center,Fujian Shishi 362700) |
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| Abstract: | According to the GenBank sequences of the duck GH gene,four pairs of specific primers were designed.The length of 4 650 bp DNA sequence was amplified from the genomic DNA abstracted from black muscovy duck blood by PCR technique.The sequences included 4 introns and 5 exons.The result of homology analysis and clustering analysis indicated that the homology had 91.2%～98.5% similarity between black muscovy duck and other birds.The result of clustering analysis showed black muscovy duck,goose and ostich clustered a class,chick,quail and turkey clustered a class,the mouse and pig clustered a class. |
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| Keywords: | mule duck POU1F1 cloning bioinformatics analysis |
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