|
|||||
|
|
| 裳卷蛾变形孢虫(Vairimorpha ceraces sp.nov.)对家蚕、鱼类的感染性试验 | |
| 引用本文： | 刘仁华,万永继,刘强波,涂增,冯光强,张琳,冯兴无.裳卷蛾变形孢虫(Vairimorpha ceraces sp.nov.)对家蚕、鱼类的感染性试验[J].蚕业科学,2006,32(1):58-62. |
| 作者姓名： | 刘仁华 万永继 刘强波 涂增 冯光强 张琳 冯兴无 |
| 作者单位： | 1. 西南大学蚕学与生物技术学院,农业部蚕桑学重点开放实验室,重庆,400716 2. 四川省农业厅阆中蚕种场,阆中,637100 3. 西南大学水产与水文学院,重庆,400716 |
| 基金项目： | 国家自然科学基金项目(编号30271006) |
| 摘 要： | 裳卷蛾变形孢虫(Vairim orpha ceracessp.nov.)对家蚕为敏感性感染,感染中量(IC50)为1.2×104,可广泛寄生各种组织,其中丝腺与马氏管被严重寄生,病蛾胚传率为2.69%,孢子发育形态明显偏向二孢子母细胞(d is-porous)发育。以2×107~4×107粒孢子/mL对每尾红鲤(red carp)添食0.5 mL,饲养40 d后解剖全部鱼体,约有10%~20%的试验鱼出现了明显的组织病变,在寄生组织中观察到大量的梨形未成熟孢子和少量的成熟孢子,但未观察到八孢子囊(octosporous)形态,病变组织疑似孢子免疫荧光检测为阳性,3次独立重复试验均得到了同样的结果,表明高浓度裳卷蛾变形孢虫可能对红鲤发生了机会性感染。试验结果对探讨微孢子虫的感染与感染适应性机制具有重要意义。 |
| 关 键 词： | 裳卷蛾变形微孢子虫 家蚕 红鲤 感染性 |
| 文章编号： | 0257-4799(2006)01-0058-05 |
| 修稿时间： | 2005年9月6日 |
Infectious Experiment of Microsporidium, Vairimorpha ceraces sp.nov. From Cerace stipatana to Silkworm (Bombyx mori) and Fish (Red Carp:Cyprinus carpio L. red Var.) |
|
| LIU Ren-Hua,WAN Yong-Ji,LIU Qiang-Bo,TU Zeng,FENG Guang-Qiang,ZHANG Lin,FENG Xing-Wu.Infectious Experiment of Microsporidium, Vairimorpha ceraces sp.nov. From Cerace stipatana to Silkworm (Bombyx mori) and Fish (Red Carp:Cyprinus carpio L. red Var.)[J].Acta Sericologica Sinica,2006,32(1):58-62. | |
| Authors: | LIU Ren-Hua WAN Yong-Ji LIU Qiang-Bo TU Zeng FENG Guang-Qiang ZHANG Lin FENG Xing-Wu |
| Abstract: | The Microsporidium,Vairimorpha ceraces sp.nov.was isolated from Cerace stipatana walker(Lepidoptera) by us and the related results were published in Acta Zootaxonomica Sinica in 2005.In this paper,we tested whether Vairimorpha ceraces had infection to other hosts,silkwom and red carp.Vairimorpha ceraces had infection to silkworm and parasitized abroad to many tissues,especially to silkgland and malpighian tube,IC_(50) was 1.2×10~(4) and transovarial transmission rate of infection moths was only 2.69%,and sporogony present octosporoblast form but many changed to disporous form in silkworm.Each red carp(Cyprimus carpio L.red Var.)was fed with 0.5 mL of 2×10~(7)～4×10~(7) spores/mL.Forty days later,about 10%~20% of the treated fish had distinct pathological tissues in which many premature spores as pear and a little mature spores were observed,but no octosporous.The suspected spores showed positive by immunofluorescence detection. 3 independent repeats tests appeared the similar result.This suggested that the high concentration of Vairimorpha ceraces spores probably led to the opportunistic infection to Cyprimus carpio L.red Var.The above results supplied the important information for studying infection and adaptability mechanism of microsporidia. |
| Keywords: | Microsporidium Vairimorpha ceraces Bombyx mori Cyprimus carpio L red Var Infection |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |