| 血管紧张素II对3T3-L1前脂肪细胞分化的影响 |
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| 引用本文： | 张琨,李世敏,张丽君,彭光华,张声华.血管紧张素II对3T3-L1前脂肪细胞分化的影响[J].华中农业大学学报,2008,27(1):131-134. |
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| 作者姓名： | 张琨 李世敏 张丽君 彭光华 张声华 |
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| 作者单位： | [1]华中农业大学食品科技学院 ,武汉430070 [2]深圳职业技术学院应用化学与生物技术学院, 深圳518055 |
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| 基金项目： | 广东省社会发展公共项目及深圳市科技基金项目(2006813501004)资助 |
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| 摘 要： | 为探讨血管紧张素II（angiotensin II,AngII）对3T3-L1前脂肪细胞分化的影响,采用鸡尾酒法诱导3T3-L1前脂肪细胞分化,并用油红O染色法和还原型烟酰胺嘌呤二核苷酸（nicotinamide-adenine dinuceotid,NADH）氧化速率法检测了3T3-L1脂肪细胞分化过程中胞浆脂质的累积和甘油磷酸脱氢酶（glycerol phosphatedehydrogenase,GPDH）的活性。结果表明,100 nmol/L的AngII可显著增大3T3-L1前脂肪细胞分化过程中胞浆脂质累积和GPDH活性（P〈0.01）,外源性AngII可促进3T3-L1前脂肪细胞的分化,增加脂肪细胞中脂类合成和储存。
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| 关 键 词： | 血管紧张素II 3T3-L1 脂肪细胞 分化 |
| 文章编号： | 1000-2421(2008)01-0131-04 |
| 修稿时间： | 2007年5月17日 |
Effects of Angiotensin II on Preadipocyte Differentiation in 3T3-L1 Cells |
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| ZHANG Kun LI Shi-min ZHANG Li-jun PENG Guang-hua ZHANG Sheng-huaCollege of Food Science , Technology,Huazhong Agricultural University,Wuhan ,China,College of Applied Chemistry , Biotechnology,Shenzhen Polytechnic College,Shenzhen ,China.Effects of Angiotensin II on Preadipocyte Differentiation in 3T3-L1 Cells[J].Journal of Huazhong Agricultural University,2008,27(1):131-134. |
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| Authors: | ZHANG Kun LI Shi-min ZHANG Li-jun PENG Guang-hua ZHANG Sheng-huaCollege of Food Science Technology Huazhong Agricultural University Wuhan China College of Applied Chemistry Biotechnology Shenzhen Polytechnic College Shenzhen China |
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| Institution: | ZHANG Kun1) LI Shi-min2) ZHANG Li-jun2) PENG Guang-hua1) ZHANG Sheng-hua1)(1)College of Food Science , Technology,Huazhong Agricultural University,Wuhan 430070,China,2)College of Applied Chemistry , Biotechnology,Shenzhen Polytechnic College,Shenzhen 518055,China) |
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| Abstract: | The effect of angiotensin II(AngII) on differentiation of 3T3-L1 preadipocyte was investigated to understand the internal relationship between obesity and hypertension.The 3T3-L1 cells were differentiated by the cocktail method,the intracytoplasmic lipids and the activity of glycerol-3-phosphate dehydrogenase(GPDH) in differentiated adipocytes were determined respectively by oil red O staining and measuring the oxidation rate of nicotinamide-adenine dinuceotid(NADH).The results showed that 100 nmol/L AngII ... |
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| Keywords: | angiotensin II 3T3-L1 adipocyte differentiation |
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