| 美味侧耳五种酶的同工酶谱在不同菌体组织中的变化 |
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| 引用本文： | 程南升,潘学仁.美味侧耳五种酶的同工酶谱在不同菌体组织中的变化[J].中国食用菌,1995(6). |
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| 作者姓名： | 程南升 潘学仁 |
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| 作者单位： | 东北林业大学林学系 |
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| 摘 要： | 比较了美味侧耳不同菌体组织中５种酶的同工酶谱变化。酯酶（Ｅ５Ｔ）和苹果酸脱氢酶（ＭＤＨ）在子实体中的酶带数和活性均高于液培菌丝。过氧化物酶（ＰＯＤ）的情况则正好相反。乙醇脱氢酶（ＡＤＨ）和山梨醇脱氢酶（ＳＯＤＨ）的酶带只在子实体中发现。酶谱随子实体部位而变异的进度同酶种类有关，ＰＯＤ变化较大，ＥＳＴ和ＭＤＨ较不明显，ＡＤＨ和ＳＯＤＨ没有变化。本实验结果为今后以这５种酶为生化遗传标记研究美味侧耳提供了基础依据。
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| 关 键 词： | 美味侧耳，同工酶，组织特异性 |
Isozyme Pattern Variation of Five Enzymes in Different Tissues of Pleurotus- Sapidus |
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| Cheng Dongsheng, Pan Xueren.Isozyme Pattern Variation of Five Enzymes in Different Tissues of Pleurotus- Sapidus[J].Edible Fungi of China,1995(6). |
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| Authors: | Cheng Dongsheng Pan Xueren |
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| Abstract: | Variation of isozyme patterns for five enzymes in different tissues of edible fungus PIeurotus sapidus was investigated,More isozyme bands and higher activity for esterase(EST)and malate dehydrogenase(MDH)were found in fruit body than in liquid-cultured mycelia,and the situation for perozidase(POD),however,was just on the con-trary.Activity of alcohol dehydrogenase(ADH) and sorbitol dehydrogenase(SODH) wasdetected only frops fruit body.The tissue specificity of isozyme patterns in fruit bodyvaried alnong the enzymes,most notable for POD,less for EST and MDH,and none forADH and SODH. The present test provided basis for further studies on Pleurotus sapidusconcerning the usage of these enzymes as biochemical or genetical markers. |
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| Keywords: | Pleurotus sapidus Isozyme Tissue specificity |
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