| 不同培养条件对菜用大黄组培苗玻璃化的影响 |
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| 引用本文： | 牛延斌,邵珠田,高二娟,姜立娜.不同培养条件对菜用大黄组培苗玻璃化的影响[J].黑龙江农业科学,2017(1). |
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| 作者姓名： | 牛延斌 邵珠田 高二娟 姜立娜 |
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| 作者单位： | 河南科技学院 园艺园林学院,河南 新乡,453003 |
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| 基金项目： | 河南省科技厅基础前沿资助项目，河南科技学院人才引进资助项目，国家级大学生创新训练资助项目 |
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| 摘 要： | 为预防菜用大黄玻璃化苗的产生、提高组培苗的培养质量,以菜用大黄丛生芽为外植体,以1/2 MS为基本培养基,研究了蔗糖、琼脂、6-BA对菜用大黄组培苗玻璃化现象的影响,同时测定了玻璃化苗与正常苗的生理指标差异。结果表明:适于菜用大黄组培苗最大增殖及玻璃化率最小的培养基组合是添加30g·L~(-1)蔗糖+6g·L~(-1)琼脂+1.0mg·L~(-1) 6-BA+1.0mg·L~(-1) NAA的1/2 MS培养基;正常苗的可溶性糖和可溶性蛋白含量明显高于玻璃化苗,而玻璃化苗的组织含水量比正常苗的略高。
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| 关 键 词： | 菜用大黄 组织培养 玻璃化 |
Effects of Different Conditions on Tissue Culture Vitrification of Rheum rhaponticum L. |
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| NIU Yan-bin,SHAO Zhu-tian,GAO Er-juan,JIANG Li-na.Effects of Different Conditions on Tissue Culture Vitrification of Rheum rhaponticum L.[J].Heilongjiang Agricultural Science,2017(1). |
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| Authors: | NIU Yan-bin SHAO Zhu-tian GAO Er-juan JIANG Li-na |
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| Abstract: | In order to prevent vitrification seedling of Rheum rhaponticum L.,and improve the quality of somaclone,taking Rheum rhaponticum L.buds as explants,with 1/2MS as the basic culture medium,the effects of sucrose,agar and 6-BA on the vitrification of tissue cultured seedlings were studied.Meanwhile,physiological and biochemical indexes between vitrification shoots and normal seedlings were determined and compared.The results showed that the high coefficient of proliferation and the low rate of vitrification was cultured in 1/2 MS medium supplemented with 30 g·L-1 sucrose,6 g·L-1 agar,1.0 mg·L-1 6-BA and 1.0 mg·L-1 NAA.The soluble sugar and soluble protein content of the normal seedlings was significantly higher than that of the vitrification shoots,while the water content of the tissue was slightly higher than that of the normal seedlings. |
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| Keywords: | Rheum rhaponticum L conditions of tissue culture vitrification |
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