| 污染疫苗的禽腺病毒分离和鉴定 |
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| 引用本文： | 周斌,刘华雷,曹瑞兵,陈溥言.污染疫苗的禽腺病毒分离和鉴定[J].南京农业大学学报,2004,27(3):78-80. |
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| 作者姓名： | 周斌 刘华雷 曹瑞兵 陈溥言 |
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| 作者单位： | 南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 国家 8 63高新技术发展项目 (2 0 0 2AA2 45 0 5 1) |
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| 摘 要： | 在用非免疫鸡胚增殖鸭病毒性肝炎病毒(E52株)过程中，发现种毒受到污染。采用聚乙二醇沉淀．Sephadex G100柱层析法纯化污染病毒，在电镜下发现一大小为70～80nm，无囊膜，20面体对称的病毒颗粒。挑选4条随机引物对其进行反转录PCR扩增，共扩增出3条基因片段。序列分析结果表明，与禽腺病毒(鸡胚致死孤儿病毒)基因组部分序列同源性分别高达99．5％、99．6％和99．5％，从而证明禽腺病毒污染了鸭病毒性肝炎病毒鸡胚尿囊液种毒。
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| 关 键 词： | 禽腺病毒 分离 鉴定 纯化 污染 疫苗 |
| 文章编号： | 1000-2030(2004)03-0078-03 |
| 修稿时间： | 2003年7月10日 |
Isolation and identification of avian adenovirus of contaminated vaccine |
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| ZHOU Bin,LIU Hua lei,CAO Rui bing,CHEN Pu yan.Isolation and identification of avian adenovirus of contaminated vaccine[J].Journal of Nanjing Agricultural University,2004,27(3):78-80. |
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| Authors: | ZHOU Bin LIU Hua lei CAO Rui bing CHEN Pu yan |
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| Institution: | ZHOU Bin,LIU Hua lei,CAO Rui bing,CHEN Pu yan * |
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| Abstract: | During duck viral hepatitis seed virus(E52)was reproduced by non immunitied embryo,a contaminated virus,which could infect chicken was detected.After purification by PEG6000,Sephadex G100 gel chromatography and dialysis,symmetry nonenveloped icosahedral virons with a diameter of 70 to 80 nm were found.Four random primers were selected to amplify this contaminated virus by RT PCR method,three DNA fragments,showing 99 5%,99 6% and 99 5% homologue respectively with the genome of avian adenovirus(chicken embryo orphan lethal virus)were got by the sequencing analysis.These results suggested that the used duck viral hepatitis seed virus(E52)was contaminated by avian adenovirus. |
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| Keywords: | avian adenovirus isolation purification PCR identification |
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