| 鱼类基因工程育种技术的研究—转基因野鲤虹鳟和鲫鱼的培育 |
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| 引用本文： | 沈俊宝,孙孝文,王鹏,阎学春,梁利群,白庆利.鱼类基因工程育种技术的研究—转基因野鲤虹鳟和鲫鱼的培育[J].水产学杂志,1993(1). |
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| 作者姓名： | 沈俊宝 孙孝文 王鹏 阎学春 梁利群 白庆利 |
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| 作者单位： | 黑龙江水产研究所育种研究室，黑龙江水产研究所育种研究室，黑龙江水产研究所育种研究室，黑龙江水产研究所育种研究室，黑龙江水产研究所育种研究室，黑龙江水产研究所育种研究室 |
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| 摘 要： | 本文报道了用鼠的MT启动子与牛生长激素基因的融合基因(mMT—bGH)为目的基因,通过显微注射等方法,导入鲤鱼、鲫鱼、虹鳟等的受精卵中,Dot杂交和Southern杂交检测,阳性信号率为9％,转基因鱼的个体体重与对照组出现明显的差异,其中一部分高于对照组,另一部分低于对照组。
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| 关 键 词： | 基因 工程 育种 转基因鱼 |
The Research of Breedipg Technologh In Fishery Gene Engineering-the Producting of Transgenic Common Carp Rainbow Trout and Goldfish |
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| Shen Junbao Sun Xiaowen Wang Peng Yan Xuechun Liang Liqun Bai Qingli.The Research of Breedipg Technologh In Fishery Gene Engineering-the Producting of Transgenic Common Carp Rainbow Trout and Goldfish[J].Chinese Journal of Fisheries,1993(1). |
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| Authors: | Shen Junbao Sun Xiaowen Wang Peng Yan Xuechun Liang Liqun Bai Qingli |
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| Abstract: | A DNA fragment containing mouse metalbthionein la promoter and bovine growth hormone gene was injected into cytoplasms of fertilized eggs of Common Carp,Rainbow Trout,Goldfish,and the exogeous DNA was detected in individual experiments fishes by Dot Bolt and Southern Blot analysis and find some fishes with positive signal grow faster than control and some slower.Further analysis is continued |
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| Keywords: | Gene Engineering Breed Transgenic Fish |
| 本文献已被 CNKI 等数据库收录！ |
