Plant regeneration from protoplasts of Iris germanica L. |
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| Authors: | K Shimizu T Yabuya T Adachi |
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| Institution: | (1) Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, 889-21 Miyazaki, Japan |
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| Abstract: | Summary Protoplasts were isolated enzymatically from suspension cultures derived from embryogenic calli induced by leaf base culture of Iris germanica. In protoplast culture, the effects of glucose concentration, different sugars and combinations of 2,4-D and KIN on protoplast division and colony formation were examined. N6 medium supplemented with 0.1–1 mg/l 2,4-D, 1 mg/l KIN, 200mg/l casein hydrolysate, 250 mg/l proline, 0.2 M glucose and 20 g/l agarose was suitable for protoplast division and colony formation. When colonies formed were transferred onto hormone-free MS medium, many plantlets were regenerated through somatic embryogenesis. Thus, we could establish a plant regeneration system from protoplasts of I. germanica.Contribution from the Laboratory of Plant Breeding, Faculty of Agriculture, Miyazaki University, Japan, No. 95. |
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| Keywords: | Iris germanica protoplast regeneration suspension culture embryogenic callus |
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