| 樱桃番茄的组织培养与离体快繁技术研究 |
| |
| 引用本文： | 曲雪艳,周庆红.樱桃番茄的组织培养与离体快繁技术研究[J].江西农业大学学报,2006,28(6):962-964. |
| |
| 作者姓名： | 曲雪艳 周庆红 |
| |
| 作者单位： | 江西农业大学,农学院,江西,南昌,330045 |
| |
| 基金项目： | 江西农业大学校青年基金项目(1545) |
| |
| 摘 要： | 以樱桃番茄(Lycopersicon esculuntumvar.Cerasiform e)的下胚轴、子叶为外植体,在附加不同质量浓度6-BA和NAA的1/2 MS培养基上进行培养。实验结果显示:消毒方法对种子发芽率的影响很大,以10%NaC lO消毒20 m in最为有效,且能够保证种子的最大发芽率。樱桃番茄下胚轴和子叶的最佳不定芽诱导培养基分别为1/2 MS 3.0 mg/L 6-BA 0.2 mg/L NAA和1/2 MS 2.0 mg/L 6-BA 0.1 mg/L NAA。最佳增殖培养基为1/2 MS 2.0 mg/L 6-BA 0.1 mg/L NAA,其增殖系数为3.4。诱导生根培养基则以1/2 MS 0.1 mg/L NAA最好。
|
| 关 键 词： | 樱桃番茄 组织培养 离体快繁 |
| 文章编号： | 1000-2286（2006）06-0962-03 |
| 收稿时间： | 2006-08-28 |
| 修稿时间： | 2006年8月28日 |
A Study on Tissue Culture and Rapid Propagation of Cherry Tomato |
| |
| QU Xue-yan,ZHOU Qing-hong.A Study on Tissue Culture and Rapid Propagation of Cherry Tomato[J].Acta Agriculturae Universitis Jiangxiensis,2006,28(6):962-964. |
| |
| Authors: | QU Xue-yan ZHOU Qing-hong |
| |
| Institution: | College of agronomy, JXAU, Nanchang 330045, China |
| |
| Abstract: | Two kinds of tissue of Lycopersicon esculuntum var.Cerasiforme,hypocotyl and cotyledon were cultured in 1/2 MS with 6-BA and NAA at different levels.The results showed that sterilization had great effect on seed germination.The sterilization with 10% NaClO solution for 20 mins had the greatest effect,which ensured the highest germination percentage.The optimal media for induction of buds of hypocotyl and cotyledon were 1/2 MS 3.0 mg/L 6-BA 0.5 mg/L NAA and 1/2 MS 2.0 mg/L 6-BA 0.1 mg/L NAA.The proliferation medium for shoots was 1/2 MS 2.0 mg/L 6-BA 0.1 mg/L NAA.The rate of proliferation of shoots was 3.4.The best medium for roots generation was 1/2 MS 0.1 mg/L NAA. |
| |
| Keywords: | Lycopersicon esculuntum var Cerasiforme tissue culture rapid in vitro propagation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
