| 利用卡那霉素筛选野罂粟转基因植株的技术研究 |
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| 引用本文： | 雒淑珍,魏玉杰,赵继荣,常英,李彦荣.利用卡那霉素筛选野罂粟转基因植株的技术研究[J].广东农业科学,2010,37(2):170-171. |
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| 作者姓名： | 雒淑珍 魏玉杰 赵继荣 常英 李彦荣 |
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| 作者单位： | 1. 甘肃省农垦农业研究院特种药材研究所,甘肃,武威,733006
2. 甘肃省农垦农业研究院天然色素作物研究所,甘肃,武威,733006 |
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| 基金项目： | 甘肃省科技厅项目(098TTCH002、0708NKCH076) |
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| 摘 要： | 待供试转基因和非转基因野罂粟植株长至5～7片叶时,用微型喷雾器将不同浓度的卡那霉素溶液均匀喷施于叶片上,结果显示浓度为6.5g/L的处理表现效果最好。对于大田筛选转基因野罂粟,在苗期对叶片喷施6.5g/L卡那霉素溶液喷雾,并对产生黄斑的野罂粟植株进行剔除后,拔节期用7.5g/L卡那霉素溶液连续5d涂抹后,对产生黄斑的植株进行再次剔除,最终得到30株未见黄斑的野罂粟植株,经PCR进行检测后,最终得到8株野罂粟转基因植株。
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| 关 键 词： | 卡那霉素 转基因 野罂粟 |
Engineering research of the wild opium poppy transgene plant by kanamycin mildew |
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| LUO Shu-zhen,WEI Yu-jie,ZHAO Ji-rong,CHANG Ying,LI Yan-rong.Engineering research of the wild opium poppy transgene plant by kanamycin mildew[J].Guangdong Agricultural Sciences,2010,37(2):170-171. |
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| Authors: | LUO Shu-zhen WEI Yu-jie ZHAO Ji-rong CHANG Ying LI Yan-rong |
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| Institution: | 1.Special Medicinal Materials Institute;Gansu State Farm Academy of Agricultural Research;Wuwei;733006;China;2.Natural Pigment Crops Institute;China |
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| Abstract: | Used different kanamycin density spurt executes on the opium poppy leaf blade with the miniature sprayer while the transgenic and the non-transgenic wild opium poppy plant growed 5 ～7 piece of leaves.The results demonstrated that the treatment of 6.5 g/L performanced was better. Wild opium poppy in field with 5～7 piece of leaves carried on the atomization, by using 6.5 g/L the kanamycin densities and yellow spot's adult plant carried on the rejection;in jointing stage carried on 5 d with 7.5 g/L kanamycin to smudge continuously, after the pair process yellow spot carried on rejects once more, 30 wild opium poppy plant without yellow spot were obtained, after PCR method examination 8 wild opium poppy transgene plant were obtained. |
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| Keywords: | kanamycin mildew transgene wild opium poppy |
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