| 蛇床子素对小菜蛾幼虫的胃毒作用及对虫体内两种酶活性的影响 |
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| 引用本文： | 孟琳琳,王进忠,张志勇,孙淑玲,张民照,张鹏飞,丁宝力,蒲媛媛.蛇床子素对小菜蛾幼虫的胃毒作用及对虫体内两种酶活性的影响[J].北京农学院学报,2010,25(3):24-27. |
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| 作者姓名： | 孟琳琳 王进忠 张志勇 孙淑玲 张民照 张鹏飞 丁宝力 蒲媛媛 |
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| 作者单位： | 北京农学院,植物科学技术学院,北京,102206;北京农学院,植物科学技术学院,北京,102206;北京农学院,植物科学技术学院,北京,102206;北京农学院,植物科学技术学院,北京,102206;北京农学院,植物科学技术学院,北京,102206;北京农学院,植物科学技术学院,北京,102206;北京农学院,植物科学技术学院,北京,102206;北京农学院,植物科学技术学院,北京,102206 |
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| 基金项目： | "大学生科学研究与创业行动计划"项目，北京市教育委员会项目，北京市自然科学基金，北京市科委项目，北京市市管高校人才强教计划资助项目，国家自然科学基金 |
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| 摘 要： | 采用生物测定和酶活力测定方法,研究了蛇床子素对敏感品系小菜蛾Plutella xylostellaL.的胃毒作用及其对小菜蛾幼虫体内谷胱甘肽-S-转移酶、乙酰胆碱酯酶活性的影响。结果表明：蛇床子素对小菜蛾幼虫具有一定的胃毒作用。2 mg/mL的蛇床子素处理萝卜苗叶片后饲喂小菜蛾幼虫12 h校正死亡率为37%,72 h时后小菜蛾幼虫的校正死亡率为93%。处理24 h时,LC50为1 521.25 mg/mL,处理48 h时,LC50为865.93 mg/mL。处理72 h时,LC50为515.5 mg/mL。不同浓度蛇床子素和不同时间处理小菜蛾幼虫后,幼虫体内的谷胱甘肽-S-转移酶和乙酰胆碱酯酶活力均产生了消长变化。经125×10-3mg/mL蛇床子素处理16 h时,小菜蛾体内的GSTs活力最高为209.15 U.mg-1prot.min-1。经125×10-3mg/mL蛇床子素处理4 h时,小菜蛾体内的AchE酶活力最高,为0.648 1 U.mg-1prot.min-1。各处理在24 h时,小菜蛾体内的GSTs活力和AchE酶活力均有明显的降低。说明蛇床子素对小菜蛾幼虫体内的谷胱甘肽-S-转移酶和乙酰胆碱酯酶活力重要的影响。
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| 关 键 词： | 蛇床子素 小菜蛾 毒力测定 谷胱甘肽-S-转移酶 乙酰胆碱酯酶 |
Stomach action of osthole and its effect on two enzyme activities of Plutella xylostella larvae |
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| MENG Lin-lin,WANG Jin-zhong,ZHANG Zhi-yong,SUN Shu-ling,ZHANG Min-zhao,ZHANG Peng-fei,DING Bao-li,PU Yuan-yuan.Stomach action of osthole and its effect on two enzyme activities of Plutella xylostella larvae[J].Journal of Beijing Agricultural College,2010,25(3):24-27. |
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| Authors: | MENG Lin-lin WANG Jin-zhong ZHANG Zhi-yong SUN Shu-ling ZHANG Min-zhao ZHANG Peng-fei DING Bao-li PU Yuan-yuan |
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| Institution: | (Department of Plant Science ＆ Technology,Beijing University of Agriculture,Beijing 102206,China) |
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| Abstract: | Stomach action of osthole to 3rd instar larvae of Plutella xylostella and its effects on activities of glutathione-S-transferase(GSTs)and acetylesterase(AChE) were studied in the laboratory.The results showed that the osthole had stomach action on the Plutella xylostella larvae.At a concentration of 2 mg/mL,the corrected mortality of the larvae was 37% at 12 h after osthole application,while the corrected mortality of larvae was 93% at 72 h.The LC50 for the 3rd instar larvae was 1 521.25 mg/mL,865.93 mg/mL and 1 521.25 mg/mL at 24 h,48 h,and 72 h,respectively.The activities of GSTs and AChE of larvae changed with concentrations modification of osthole,the highest GSTs activity of larvae treated with the dosage of 125×10-3 mg/mL of osthole was 209.15 U·mg-1prot min-1 at 12 h,while the highest AChE activities was 0.648 1 U·mg-1 prot·min-1at 4 h.While the GSTs and AChE activities decreased significantly at 24 h.These results suggested osthole could influence GSTs and AChE activities of Plutella xylostella larvae. |
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| Keywords: | osthole Plutella xylostella stomach action glutathione-S-transferase acetylesterase |
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