| 库尔勒香梨萼片脱落与宿存相关基因的差异表达分析 |
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| 引用本文： | 董芳园,张飞,王钰婷,牛建新.库尔勒香梨萼片脱落与宿存相关基因的差异表达分析[J].新疆农业科学,2013,50(1):57-64. |
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| 作者姓名： | 董芳园 张飞 王钰婷 牛建新 |
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| 作者单位： | 新疆石河子大学农学院园艺系,新疆石河子,832003 |
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| 基金项目： | 国际科技合作项目(2009YD32);国家科技基础性工作专项(2012FY110100-5-3) |
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| 摘 要： | 目的]利用mRNA差异显示(mRNA differential display PCR,DDRT-PCR)技术,寻找、筛选并确定与库尔勒香梨萼片脱落和宿存相关基因的差异表达时期以及相关的差异基因.方法]以新疆库尔勒香梨盛花前期、盛花期及其盛花后期三个时期同一花序的第2和第4朵花为试材,通过DDRT-PCR技术分离和克隆库尔勒香梨萼片脱落和宿存相关基因片段.结果]分离得到42条差异片段,其中双引物片段有7条,在GenBank中同源性比较发现有2条与控制开花以及激素调节有密切关系的SPL和MYB类转录因子有很高的同源性,分别是78;和86.83;.结论]实验为获得香梨萼片脱落与宿存差异表达基因建立的体系.为相关基因全长的克隆奠定基础,并为进一步验证其功能提供依据.
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| 关 键 词： | 库尔勒香梨 萼片 DDRT-PCR 转录因子 |
| 收稿时间： | 2013-01-25 |
Differential Expression Analysis of Calyx Falling off Korla Fragrant Pears and Their Persistent Related Genes |
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| DONG Fang-yuan,ZHANG Fei,WANG Yu-ting,NIU Jian-xin.Differential Expression Analysis of Calyx Falling off Korla Fragrant Pears and Their Persistent Related Genes[J].Xinjiang Agricultural Sciences,2013,50(1):57-64. |
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| Authors: | DONG Fang-yuan ZHANG Fei WANG Yu-ting NIU Jian-xin |
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| Institution: | DONG Fang - yuan,ZHANG Fei,WANG Yu - ting,NIU Jian - xin (Department of Horticulture,College of Agronomy,Shihezi University,Shihezi Xinjiang 832003,China) |
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| Abstract: | Objective]The expression of differential gene was studied in calyx falling - off Korla Fragrant Pears and their persistent related genes through the method of DDRT - PCR in order to seek,screen and define the genes and blooming.Method]Korla Fragrant Pear flower(the second flower and the fourth flower) of the same inflorescence of three anthesis stages(early full bloom,full bloom,the end of bloom) in the same tree were taken as material.Improved DDRT - PCR method was used to investigate the differential display of genes relate to calyx leaving and persistent related genes of Korla Fragrant Pears.Result]The result showed that the differential display exist in early full bloom stage,42 different expressed cDNA fragments were obtained and only 7 double - primer bands were amplified.The alignments of nucleotide and amino acid sequences indicated that there were 2 fragments which have high homology that might be closely related to SPL and MYB,the two translation factors important to regulation on plant phytohormone and flowering.The homology was 78%and 86.83%,respectively.Conclusion]This study had established the system of DDRT - PCR to obtain genes which relate to calyx of leaving and persistent.It laid on a foundation for cloning full length of relative gene,which also makes insurance to the further studying its physiological and biochemical characteristics and functions of thd gene expression product. |
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| Keywords: | Korla Fragrant Pear calyx DDRT-PCR translation factor |
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