| NTG诱变筛选高产柚苷酶抗药性突变株 |
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| 引用本文： | 陈玲,涂晓嵘,涂国全.NTG诱变筛选高产柚苷酶抗药性突变株[J].江西农业大学学报,2007,29(4):670-674. |
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| 作者姓名： | 陈玲 涂晓嵘 涂国全 |
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| 作者单位： | 江西农业大学,生物科学与工程学院,江西,南昌,330045 |
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| 摘 要： | 通过筛选柚苷酶产生菌3-54菌株孢子的敏感抗生素最低致死浓度,采用NTG对出发菌株孢子进行诱变处理,将诱变后的孢子涂在含纳他霉素致死浓度(2.0 U/mL)的平板筛选培养基上,获得了大量的抗药性致死突变株。再结合透明圈法,经过透明圈初筛和多次摇瓶复筛,从180个纳他霉素抗性基因突变株中筛选得到1株摇瓶发酵单位达770.06 U/mL的3-54-NTG-16号菌株,比出发菌株的产酶能力提高了近100%。
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| 关 键 词： | 柚苷酶 抗药性致死突变 透明圈 |
| 文章编号： | 1000-2286（2007）04-0670-05 |
| 收稿时间： | 2006-12-01 |
| 修稿时间： | 2006-12-012007-04-09 |
Screening of Chemical Resistant Mutant Strains with High Naringinase-producing Capacity by Means of NTC Induction |
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| CHEN Ling,TU Xiao-rong,TU Guo-quan.Screening of Chemical Resistant Mutant Strains with High Naringinase-producing Capacity by Means of NTC Induction[J].Acta Agriculturae Universitis Jiangxiensis,2007,29(4):670-674. |
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| Authors: | CHEN Ling TU Xiao-rong TU Guo-quan |
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| Institution: | Department of Bioengineering,JAU, Nanchang 330045, China |
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| Abstract: | After testing the resistance of sensitive antibiotic to naringinase-producing strains NO.3-54,a lot of chemical resistant mutants were screened after the spores regenerated on the lethal media when they were treated with NTG.Through the transparent circle screening and sever rotation-flask experiments,the high-yielding strain 3-54-NTG-16 was obtained from the 180 mutants,the productivity of which is 770.06U/mL,almost 100% higher than the original strain's in the rotation-flask experiments. |
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| Keywords: | naringinase chemical resistant lethal mutation transparent circle |
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