| 正交试验优选酸水法提取黄连中小檗碱的工艺及其抑菌活性的研究 |
| |
| 引用本文： | 郭慧琳,贺奋义,杨明.正交试验优选酸水法提取黄连中小檗碱的工艺及其抑菌活性的研究[J].畜牧兽医杂志,2013,32(4):12-14. |
| |
| 作者姓名： | 郭慧琳 贺奋义 杨明 |
| |
| 作者单位： | 1. 甘肃省动物疫病预防控制中心,甘肃兰州,730046
2. 甘肃省畜牧兽医研究所 |
| |
| 摘 要： | 采用正交试验设计,采用酸水法提取黄连中小檗碱,并测定提取的小檗碱对八株大肠埃希氏杆菌的体外最小抑菌浓度（MIC）。结果表明,最佳正交提取工艺为C3B3A1,即硫酸浓度0.2%、冷浸72h、用15%NaCl盐析,提取物对八株致病性大肠杆菌的MIC为0.50mg/mL-1.5mg/mL。提取的小檗碱对致病性大肠杆菌具有较好的抑菌作用。
|
| 关 键 词： | 黄连 小檗碱 正交提取 体外抑菌 |
Optimization of Acid-Water Method for Extracting Berberine from Rhizoma Coptis by Orthogonal Design and Study on Its Antibacterial Activity in Vitro |
| |
| GUO Hui-lin
,
HE Fen-yi
,
YANG Ming.Optimization of Acid-Water Method for Extracting Berberine from Rhizoma Coptis by Orthogonal Design and Study on Its Antibacterial Activity in Vitro[J].Journal of Animal Science and Veterinary Medicine,2013,32(4):12-14. |
| |
| Authors: | GUO Hui-lin HE Fen-yi YANG Ming |
| |
| Institution: | 1. Animal Disease Control Center in Gansu Province, Lanzhou Gansu 730046,china 2. Gansu Institute of Animal Science and Veterinary Medicine) |
| |
| Abstract: | The extracting process with acid-water method was optimized for berberine from Rhizoma Coptis by orthogonal test. The minimal inhibitory concentration（MIC）was measured by method of bacteriostasis tests in vitro with eight kinds of pathogenic Escherichia coli. The results showed that the optimum extracting process was C3B3Al,namely,0.2 % sulfuric acid, the cold--maceration for 72 h, salting out with salt at 15 %. MIC of berberine against pathogenic Escherichia coli was 0.50-1.50 mg/mL. Thus,berberine was shown to have significant inhibition on the pathogenic Escherichia coll. |
| |
| Keywords: | Rhizoma Coptidis berberine orthogonal test in vitro antibacterial activity |
| 本文献已被 维普 万方数据 等数据库收录！ |
