| 断奶及日龄对仔猪骨髓组织抗菌肽PR-39 基因表达的影响 |
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| 作者姓名： | zhaoyurong |
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| 作者单位： | 湖南农业大学动物科技学院 |
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| 摘 要： | 分别于出生当天、3、14、23、28 日龄随机屠宰杜长大公猪4头，采集骨股骨髓组织。用RT-PCR方法，以18S rRNA作内标，定量分析不同日龄仔猪骨髓组织抗菌肽PR-39 mRNA表达的差异及断奶对其表达的影响。结果表明：不同日龄仔猪骨髓组织PR-39 mRNA 相对丰度有明显的差异(P < 0.05)。出生时较低，吮吸初乳2天后显著增加(P < 0.05)，14日龄时较3日龄显著下降(P < 0.05)，随后随日龄的增加，其表达量呈上升趋势；断奶导致其显著下降(P < 0.05)。
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| 关 键 词： | 半定量RT-PCR 猪 断奶 抗菌肽 PR-39 基因表达 |
| 收稿时间： | 2007-3-12 |
| 修稿时间： | 2007-5-17 |
Effect of Age and Weanling on the PR-39 Gene Expression in Femur Marrow of Piglets |
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| zhaoyurong.Effect of Age and Weanling on the PR-39 Gene Expression in Femur Marrow of Piglets[J].Journal of Agricultural Biotechnology,2008,16(2). |
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| Abstract: | In the present investigation an optimized semi-quantitative RT-PCR was applied to detect the PR-39 gene expression difference in femur marrow of piglets at different ages and weaning by using 18S rRNA as inner marker. Samples were taken at birth ,3 ,14 , 23 ,28 days of age respectively. The results showed that age affected PR-39 mRNA levels in femur marrow significantly (P < 0.05), the expression of PR-39 mRNA was the lowest at birth, then increased remarkably at 3 days of age, but the expression at 14 d-old was lower than that of 3 days (P < 0.05),and it was increased thereafter. Weaning affected PR-39 mRNA levels significantly also (P < 0.05). Weaning decreased the PR-39 mRNA levels at the same day. |
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