| p38MAPK信号通路选择性调节FSH对甾体生成
的诱导作用研究 |
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| 引用本文： | 赵云焕,张自富,赵 聘.p38MAPK信号通路选择性调节FSH对甾体生成
的诱导作用研究[J].中国农学通报,2006,22(2):9-9. |
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| 作者姓名： | 赵云焕 张自富 赵 聘 |
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| 作者单位： | 信阳农业高等专科学校,河南,信阳,464000 |
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| 基金项目： | 国家“十五”奶类重大专项“西北农区（陕西）奶业现代化生产技术集成与产业化示范（2002BA518A17）” |
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| 摘 要： | 利用乙烯雌酚（DES）处理23日龄SD大鼠，分离卵巢颗粒细胞(GC)进行无血清培养。结果表明，FSH (50ng/m1) 处理GC，可迅速激活有丝分裂原蛋白激酶（p38 MAPK），FSH处理5min便可观察到磷酸化的p38 MAPK；FSH处理30min，磷酸化的p38 MAPK水平达到最高;向培养液中加入H89(蛋白激酶A抑制剂，10μM)，则显著抑制了FSH对p38 MAPK的激活作用，提示这种激活作用依赖于蛋白激酶A(PKA)。用 SB203580(p38MAPK抑制剂，20μM)抑制p38 MAPK激活，则进一步提高了FSH对孕酮和甾体生成快速调节蛋白（StAR）的诱导作用，同时降低了FSH对雌激素生成的促进作用(p<0.01)。RT-PCR结果显示：抑制p38 MAPK活性后，FSH对StAR mRNA刺激作用明显增强，但对细胞色素P450芳香化酶（P450arom）mRNA的诱导作用却减弱了(p<0.05)。激光共聚焦和蛋白印迹结果显示：在GC中，StAR蛋白主要分布在线粒体中；与对照组相比，FSH显著提高了StAR的荧光强度和蛋白水平；抑制p38MAPK活性则增强了FSH对StAR蛋白表达的诱导作用。
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| 关 键 词： | FSH GC MAPK StAR |
| 收稿时间： | 2005-09-23 |
| 修稿时间： | 2005-09-232005-11-10 |
Study on FSH-activated p38MAPK Pathway Differently Regulated the Progesterone and Estrogen Synthesis in the Cultured Primary GCs |
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| Zhao Pin,Zhang Zifu,Zhao Yunhuan.Study on FSH-activated p38MAPK Pathway Differently Regulated the Progesterone and Estrogen Synthesis in the Cultured Primary GCs[J].Chinese Agricultural Science Bulletin,2006,22(2):9-9. |
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| Authors: | Zhao Pin Zhang Zifu Zhao Yunhuan |
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| Institution: | Xinyang Agricul Turae College, Xinyang Henan 464000 |
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| Abstract: | GCs were obtained from the ovaries of activity of DES-treated immature rats and cultured in serum-free medium. FSH receptor occupancy lesded to phosphorylation/activation of p38 MAPK in time-dependent manner. Phosphorylated p38 MAPK by FSH was observed within 5 min and reached the highest level at 30 min. Moreover, such activation was protein kinase A-dependent as indicated by the results using specific inhibitors.Interestingly.inhibition of p38 MAPK acitivity with SB203580 augmented estradiol production at the same time (p<0.01). RT-PCR data showed that inclusion of SB203580 in the FSH-stimulated FSH-induced STAR mRNA Production, while decreased the FSH-stimulated P450arom mRNA expression of STAR in mitochondria than FSH treatment alone. |
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| Keywords: | FSH GC MAPKs StAR |
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