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“中宁小枣”组培快繁技术研究
引用本文:柳金凤,李永华,伍会萍.“中宁小枣”组培快繁技术研究[J].北方园艺,2012(16):98-100.
作者姓名:柳金凤  李永华  伍会萍
作者单位:宁夏林业研究所股份有限公司,种苗生物工程国家重点实验室,宁夏银川750004;宁夏林业研究所股份有限公司,种苗生物工程国家重点实验室,宁夏银川750004;宁夏林业研究所股份有限公司,种苗生物工程国家重点实验室,宁夏银川750004
摘    要:以宁夏"中宁小枣"种仁为外植体,进行了愈伤组织、不定芽诱导、分化、生根试验研究。结果表明:MS+6-BA 1.0mg/L+NAA 1.0mg/L为最适合种子萌芽诱导的培养基;MS+KT 2.0mg/L+IBA 1.0mg/L+GA31.0mg/L为最佳试管苗分化的培养基;IBA和NAA能明显促进难繁植物生根反应,1/2MS+IBA 0.8mg/L+NAA 0.2mg/L为最适合试管苗生根培养基。

关 键 词:枣树  不定芽  组织培养

Study on Tissue Culture and Rapid Propagation Technology of Ziziphus jujuba Mill
LIU Jin-feng , LI Yong-hua , WU Hui-ping.Study on Tissue Culture and Rapid Propagation Technology of Ziziphus jujuba Mill[J].Northern Horticulture,2012(16):98-100.
Authors:LIU Jin-feng  LI Yong-hua  WU Hui-ping
Institution:(The State Key Laboratory of Seedling and Bioengineering,Ningxia Forestry Institute,Yinchuan,Ningxia 750004)
Abstract:Taking Ziziphus jujuba acinus as explants,the induction of the buds,differentiation,taking roots and transplants were studied.The results showed that MS+6-BA 1.0 mg/L+NAA 1.0 mg/L was the most suitable medium for the induction of adventitious buds.MS+KT 2.0 mg/L+IBA 1.0 mg/L+GA3 1.0 mg/L was the most suitable medium for differentiation.IBA and NAA promoted root formation,1/2MS+IBA 0.8 mg/L+NAA 0.2 mg/L was the most suitable condition for inducing roots.
Keywords:Ziziphus jujuba Mill  adventitious buds  tissue culture
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