| 北马兜铃营养生长期过氧化物酶同工酶分析 |
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| 引用本文： | 孙睿,邵红,刘娟,王红康,于洋.北马兜铃营养生长期过氧化物酶同工酶分析[J].北方园艺,2012(16):88-89. |
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| 作者姓名： | 孙睿 邵红 刘娟 王红康 于洋 |
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| 作者单位： | 佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学药学院,黑龙江佳木斯,154007 |
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| 基金项目： | 佳木斯大学大学生科技创新资助项目 |
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| 摘 要： | 用聚丙烯酰胺垂直板凝胶电泳技术对北马兜铃营养生长期根、幼茎、老茎、叶柄、未展开幼叶、展开幼叶和成熟叶的过氧化物酶(POD)同工酶进行了分析。结果表明:北马兜铃营养生长期7个组织器官共有10条POD酶带,其POD同工酶具有组织特异性,其中未展开幼叶的POD同工酶酶带丰富、活性较强、含量较高,是POD同工酶分析最好的材料,而成熟叶片由于POD酶种类太少,最不适合作POD同工酶分析。
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| 关 键 词： | 北马兜铃 营养生长期 过氧化物酶 同工酶 |
Study on POD Isoenzymes of Aristolochia contorta Bunge at Vegetative Growth Phase |
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| SUN Rui
,
SHAO Hong
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LIU Juan
,
WANG Hong-kang
,
YU Yang.Study on POD Isoenzymes of Aristolochia contorta Bunge at Vegetative Growth Phase[J].Northern Horticulture,2012(16):88-89. |
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| Authors: | SUN Rui SHAO Hong LIU Juan WANG Hong-kang YU Yang |
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| Institution: | 1(1.College of Life Science,Jiamusi University,Jiamusi,Heilongjiang 154007;2.College of Pharmacy,Jiamusi University,Jiamusi,Heilongjiang 154007) |
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| Abstract: | The POD enzymes from root,tender stem,old stem,petiole,unexpanded tender leaf,expanded tender leaf and mature leaf of Aristolochia contorta Bunge at vegetative growth phase were analyzed by vertical polyacrylamide gel electrophoresis(PAGE).The results showed that 10 bands of POD isoenzymes from 7 different tissues and organs of Aristolochia contorta Bunge at vegetative growth phase were expressed and POD isoenzymes had a tissue specificity.Unexpanded tender leaf which had abundant enzyme bands,stronger activity and higher content were the good materials for analysis on POD isoenzyme.There were the fewest enzyme bands in mature leaf,so it was not fit for analysis on POD isoenzyme. |
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| Keywords: | Aristolochia contorta Bunge vegetative growth phase POD isoenzyme |
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