| 叶用莴苣离体培养和植株再生 |
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| 引用本文： | 朱路英,刘玲,孟祥栋,张振贤.叶用莴苣离体培养和植株再生[J].园艺学报,2002,29(2):181-184. |
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| 作者姓名： | 朱路英 刘玲 孟祥栋 张振贤 |
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| 作者单位： | (山东农业大学园艺系, 泰安271018 ; 北京市农林科学院蔬菜研究中心, 北京100089 ; 烟台师范学院生物系, 烟台264025) |
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| 摘 要： | 以叶用莴苣( Lactuca sativa L. ) 4 个栽培品种的子叶和真叶为材料进行诱导芽分化及生根成苗试验, 从中选出较理想的品种和培养基配方, 建立了我国叶用莴苣栽培品种的快速、高频离体植株再生体系。外植体分化频率可达100 % , 平均每外植体分化不定芽5. 6 个。3 d 苗龄的子叶分化效果最佳。从子叶外植体培养至形成完整小植株仅需30～35 d。
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| 关 键 词： | 叶用莴苣 离体培养 植株再生 组织培养 |
| 文章编号： | 0513-353X(2002)02-0181-02 |
| 修稿时间： | 2001年4月9日 |
In Vitro Shoot Differentiation from Cotyledon Explants of Lettuce |
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| Zhu Luying ,Liu Ling ,Meng Xiangdong ,and Zhang Zhenxian.In Vitro Shoot Differentiation from Cotyledon Explants of Lettuce[J].Acta Horticulturae Sinica,2002,29(2):181-184. |
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| Authors: | Zhu Luying Liu Ling Meng Xiangdong and Zhang Zhenxian |
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| Institution: | 1. epartment of Pathology, Medical College of Fudan University, Shanghai 200032, China;
2. Department of Pathology, Beijing University of Chinese Medicine, Beijing100029, China |
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| Abstract: | An efficient and fast method for shoot differentiation from cotyledon explants of lettuce ( Lactuca sativa L.)was developed. Shoot differentiation rate of 2 lettuce cultivars reached as high as 100%, each explant produced up to 5-6 seedlings. Factors influencing in vitro explant differentiation were studied. The shoot differentiation and enlongation rate were improved by using cotyledon of three days as explant. The regeneration period from lettuce cotyledon explants to a seedling was shortened to 30-35 days. |
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| Keywords: | Lactuca sativa L Tissue culture Organogensis |
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