| 5种禽类卵黄低密度脂蛋白对猪精子冷冻效果的影响 |
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| 引用本文： | 吕瑞凯,胡建宏,王 红,程 亮,江中良,李青旺,姚 俊,张鹏飞.5种禽类卵黄低密度脂蛋白对猪精子冷冻效果的影响[J].西北农业学报,2011,20(9):5-10. |
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| 作者姓名： | 吕瑞凯 胡建宏 王 红 程 亮 江中良 李青旺 姚 俊 张鹏飞 |
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| 作者单位： | 西北农林科技大学动物科技学院 |
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| 基金项目： | 陕西省优秀博士学位论文科研资助项目(A213020901);西北农林科技大学科研专项(07ZR002) |
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| 摘 要： | 为了确定具有最佳抗冷冻效果的禽类卵黄低密度脂蛋白(LDL)及其添加质量分数,在猪精液冷冻稀释液中分别添加质量分数为6%、7%、8%、9%和10%的鸡、鸭、鹌鹑、鸽子和鸵鸟的卵黄LDL,分析不同禽类的LDL对猪精子的冷冻保存效果。结果表明,稀释液中添加质量分数为9%的鸡、鸭、鹌鹑、鸽子卵黄LDL以及质量分数8%的鸵鸟卵黄LDL时,冷冻-解冻后精子活率最高,分别达到42.33%、35.63%、31.47%、47.33%和36.40%。以5种禽类LDL最佳质量分数配制冷冻稀释液冷冻精子,发现质量分数为9%的鸽蛋LDL冻存猪精子时解冻后精子活率达到47.33%,顶体完整性达到62.57%,质膜完整性达到48.13%,均显著优于其他处理组(P<0.05)。说明鸽子卵黄LDL对猪精子具有良好的冷冻保护性能,可提高猪精子抵抗低温打击的能力。
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| 关 键 词： | 猪精子 冷冻保护 禽类卵黄 LDL |
Comparative Study of LDL Extracted from Five Avian |
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| Ruikai,HU Jianhong,WANG Hong,CHENG Liang,JIANG Zhongliang,LI Qingwang,YAO Jun and ZHANG Pengfei.Comparative Study of LDL Extracted from Five Avian[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(9):5-10. |
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| Authors: | Ruikai HU Jianhong WANG Hong CHENG Liang JIANG Zhongliang LI Qingwang YAO Jun and ZHANG Pengfei |
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| Institution: | (College of Animal Science and Technology,Northwest A&F University,Yangling Shaanxi 712100) |
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| Abstract: | Low density lipolipid(LDL) can protect sperm from freezing damage during the sperm frozen-thawed process.In order to distinguish the efficiency of LDL extracted from different avian species,to confirm which one and how much of it has the best anti-freezing effect,LDL extracted from eggs of hen,duck,quail,pigeon and ostrich with the mass fraction of 6%,7%,8%,9% and 10% was added into the cryopreservation diluents of boar sperm separately.The result showed that after the frozen-thawed process,LDL extracted from eggs of hen,duck,quail and pigeon with concentration of 9% and ostrich LDL with concentration of 8% could protect sperm with highest livability of 42.33%,35.63%,31.47%,47.33% and 36.40%,respectively.They were significantly better than that of other concentrations(P<0.05).Among the cryopreservation diluents and cryopreservation sperm prepared with the five avian species with the optimum concentration,it was found that the 9% pigeon LDL group showed 47.33% sperm livability,62.57% acrosome integrity and 48.13% membrane integrity after thawing,all of them were better than that of other groups(P<0.05).Based on the most significant increase of the cold resistance ability that conferred by pigeon egg yolk LDL,it has a good performance on sperm cryopreservation. |
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| Keywords: | Boar sperm Cryopreservation Avian egg yolk Low density lipolipid(LDL) |
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