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广西香蕉主要叶斑病菌拮抗微生物的分离筛选
引用本文:付岗,林善海,黄思良,周双全,汪茜,岑贞陆.广西香蕉主要叶斑病菌拮抗微生物的分离筛选[J].西南农业学报,2007,20(3):421-424.
作者姓名:付岗  林善海  黄思良  周双全  汪茜  岑贞陆
作者单位:1. 广西农业科学院植物保护研究所,广西,南宁,530007;广西大学农学院,广西,南宁,530005
2. 广西大学农学院,广西,南宁,530005
3. 长江大学农学院,湖北,荆州,434025
基金项目:广西农业科学院科技发展基金(2005006)
摘    要:从不同作物根际土壤中分离得到微生物菌株1223株。以香蕉叶斑病5种病原菌为靶标进行的拮抗菌筛选结果显示,香蕉尾孢的拮抗菌检出率(39.3%)最低。在以香蕉尾孢为指示菌的筛选中发现,放线菌中拮抗性菌株的出现率(33.3%)明显高于细菌(14.9%),不同类别作物中以香蕉根围拮抗性菌株的出现率(23.3%)最高。筛选到的拮抗细菌B106菌株和拮抗放线菌St1607菌株连续移植6次,对香蕉尾孢的抑菌力均保持稳定。2株拮抗菌除对香蕉叶斑病菌具较高抑菌活性外,对其余11种植物病原菌均表现不同程度的抑制效果。

关 键 词:香蕉叶斑病  香蕉尾孢  拮抗微生物  筛选
文章编号:1001-4829(2007)03-0421-04
修稿时间:2007-01-13

Isolation and screening of antagonistic microorganisms against pathogens of banana leaf spot diseases in Guangxi
FU Gang,LIN Shan-hai,HUANG Si-liang,ZHOU Shuang-quan,WANG Qian,CEN Zhen-lu.Isolation and screening of antagonistic microorganisms against pathogens of banana leaf spot diseases in Guangxi[J].Southwest China Journal of Agricultural Sciences,2007,20(3):421-424.
Authors:FU Gang  LIN Shan-hai  HUANG Si-liang  ZHOU Shuang-quan  WANG Qian  CEN Zhen-lu
Institution:1. Institute of Plant Protection,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007, China ;2. College of Agriculture,Yangtze University, Guangxi Nanning 530005, China ;3. College of Agriculture, Changjiang University, Hubei Jingzhou 434025, China
Abstract:Totally 1223 strains were isolated from different crops rhizosphere.The antagonistic abilities of all the isolated strains against 5 pathogens(Cercospora musae,Cordana musae,Helminthosporium torulosa,Alternaria musae,Curvularia lunata) of banana leaf spot diseases were tested on PDA plates.The result showed that the occurrence frequency(39.3 %) of antagonistic strains against C.musae was lowermost.The percentage of the strains with suppressive ability against C.musae in actinomycete group was higher than that in bacterium group.The occurrence frequency(23.3 %) of antagonistic strains from banana rhizosphere was higher than that from other crops.No significant change in suppressive ability of strains B106 and St1607 was detected during 6 times continuous transfers on artificial medium.A broad suppressive spectrum of the two antagonists against other plant pathogens was confirmed.
Keywords:banana leaf spot disease  Cercospora musae  antagonistic microorganism  screening
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