| 锌对小麦抗氧化酶活性的影响 |
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| 引用本文： | 王培,孙倩,魏述勇,苗明升.锌对小麦抗氧化酶活性的影响[J].安徽农业科学,2010,38(12):6398-6399. |
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| 作者姓名： | 王培 孙倩 魏述勇 苗明升 |
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| 作者单位： | 山东师范大学生命科学学院,山东济南,250014 |
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| 摘 要： | 目的]探讨内源抗氧化酶系统在作物抵抗外源重金属胁迫中的作用。方法]用不同浓度锌溶液处理盆栽小麦幼苗,至幼苗生长缓慢时取材测定过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）等指标。结果]20、50、100、200、400和800mg/L锌溶液处理的小麦POD活性分别比对照组提高了54.8%、58.2%、115.2%、157.8%、408.5%和564.0%。200、400和800mg/L锌溶液处理的小麦SOD活性分别是对照组的77.1%、68.4%和61.2%。20、50、100、200和400mg/L锌溶液处理的小麦CAT活性分别比对照组提高了1.7%、4.0%、13.2%、16.3%和22.8%。20和50mg/L锌溶液处理的小麦的丙二醛含量分别为对照的96.4%和97.5%。100、200、400和800mg/L锌溶液处理的小麦的丙二醛含量分别比对照组提高了57.7%、92.7%、235.4%和464.4%。结论]在锌胁迫下的小麦活性氧清除系统中,POD是关键的抗氧化酶。
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| 关 键 词： | 小麦 锌 抗氧化酶 丙二醛 |
Effect of Zinc on the Antioxidase Activity in Wheat |
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| Institution: | WANG Pei et al(College of Life Science,Shandong Normal University,Jinan,Shandong 250014) |
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| Abstract: | Objective] The purpose of this research was to discuss the action of endogenous antioxidase system in resistance of crops to exogenous heavy metal stress.Method] The potted wheat seedlings were treated with different concn.of zinc(Zn2+)solution till the seedlings grew slowly,then the materials were fetched for determining the indexes,such as peroxidase(POD),superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA).Result] Compared with CK group,the POD activities in wheat treated with 20,50,100,200,400 and 800 mg/L Zn2+ solution were enhanced by 54.8%,58.2%,115.2%,157.8%,408.5% and 564.0% resp.;the SOD activities in wheat treated with 200,400 and 800 mg/L Zn2+ solution were 77.1%,68.4% and 61.2% resp.;the CAT activities in wheat treated with 20,50,100,200 and 400 mg/L Zn2+ solution were enhanced by 1.7%,4.0%,13.2%,16.3% and 22.8% resp.;the MDA contents in wheat treated with 20 and 50 mg/L Zn2+ solution were 96.4% and 97.5% resp.;the MDA contents in wheat treated with 100,200,400 and 800 mg/L Zn2+ solution were enhanced by 57.7%,92.7%,235.4% and 464.4% resp.Conclusion] In the scavenging system of active oxygen in wheat under Zn2+ stress,POD was key antioxidase. |
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| Keywords: | Wheat Zinc Antioxidase Malondialdehyde |
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