| 菊花的快速繁殖 |
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| 引用本文： | 焦德志,李波,魏明丽,马秋艳,孙琦.菊花的快速繁殖[J].北方园艺,2007(6):208-210. |
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| 作者姓名： | 焦德志 李波 魏明丽 马秋艳 孙琦 |
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| 作者单位： | 1. 黑龙江省齐齐哈尔大学生命科学与工程学院,161006
2. 黑龙江省齐齐哈尔市种子站,161000 |
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| 摘 要： | 以带顶芽的菊花茎段为外植体,接种在诱导侧芽生长培养基中(MS NAA0.2mg/L 6-BA2.0mg/L),25d左右诱导成芽,再将芽接种于愈伤组织诱导培养基中(MS NAA0.1mg/L 6-BA3.0mg/L)进行继代培养,最后接种于生根培养基中(1/2MS NAA0.1mg/L),15d即可生根,30～35 d平均生根率达100%.将生根试管苗开盖练苗,移栽,成活率达95%以上.
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| 关 键 词： | 菊花 组织培养 快速繁殖 |
| 文章编号： | 1001-0009（2007）06-0208-03 |
| 收稿时间： | 2007-01-23 |
| 修稿时间： | 2007-01-23 |
Rapid Propagation of Chrysanthemum |
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| Jiao De-zhi,Li Bo,Wei Ming-li,Ma Qiu-yan,Sun Qi.Rapid Propagation of Chrysanthemum[J].Northern Horticulture,2007(6):208-210. |
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| Authors: | Jiao De-zhi Li Bo Wei Ming-li Ma Qiu-yan Sun Qi |
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| Institution: | 1. College of Life Science and Technology, Qiqihaer University, Heilongiiang 161006;2. Seed Station of Qiqihaer, Heilongjiang 161000 |
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| Abstract: | The stem segments of chrysanthemum with terminal buds were used as explants and inoculated into the medium(MS 0.2mg/L NAA 2.0 mg/L 6-BA) which could induce the growth of lateral buds.The explants were induced to be buds after 25 days.Then the buds were subcultured into the callus-inducing medium(MS 0.1mg/LNAA 3.0mg/L6-BA).At last the callus were inoculated into the root-inducing medium(1/2MS 0.1mg/L NAA).The roots were induced in the following 15 days.The average rate of rooting was up to 100% during 30~35 days.The plantlets of chrysanthemum were domesticated in culture bottles without covers,then transplanted.The survival rate of them was above 95%. |
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| Keywords: | Chrysanthemum Tissue culture Rapid propagation |
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