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| 不同分化培养基对秋水仙碱处理大葱愈伤组织的影响 | |
| 引用本文： | 高莉敏,宗红,陈运起,高秀云,董飞.不同分化培养基对秋水仙碱处理大葱愈伤组织的影响[J].山东农业科学,2011(2):27-29. |
| 作者姓名： | 高莉敏 宗红 陈运起 高秀云 董飞 |
| 作者单位： | 1. 山东省农业科学院蔬菜研究所,山东,济南,250100 2. 成武县林业局,山东,成武,274200 3. 山东农业大学园艺科学与工程学院,山东,泰安,271018 |
| 基金项目： | 山东省农业科学院创新基金项目，山东省良种产业化项目 |
| 摘 要： | 采用正交设计,测定了不同分化培养基对秋水仙碱处理大葱愈伤组织的影响。结果表明,在不添加2,4-D的基础上,适当提高蔗糖浓度,可以减缓愈伤组织褐化现象的发生,提高芽分化率,以处理6（蔗糖40 g/L＋琼脂15 g/L＋6-BA 1.5 mg/L）加倍诱导率最高。试验得到了两种嵌合体植株,一种为同时具有三倍体和二倍体细胞的植株,另一种为同时具有四倍体和二倍体细胞的植株。 |
| 关 键 词： | 大葱 愈伤组织 分化培养基 |
Effects of Different Differentiation Media on Welsh Onion Callus |
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| GAO Li-min,ZONG Hong,CHEN Yun-qi,GAO Xiu-yun,DONG Fei.Effects of Different Differentiation Media on Welsh Onion Callus[J].Shandong Agricultural Sciences,2011(2):27-29. | |
| Authors: | GAO Li-min ZONG Hong CHEN Yun-qi GAO Xiu-yun DONG Fei |
| Institution: | GAO Li-min1,ZONG Hong1,CHEN Yun-qi1,GAO Xiu-yun2,DONG Fei3(1.Vegetable Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China,2.Forestry Bureau of Chengwu County,Chengwu 274200,3.College of Horticulture Science and Engineering,Shandong Agricultural University,Taian 271018,China) |
| Abstract: | The effects of different differentiation media on welsh onion callus treated with colchicin were determined by the orthogonal design experiment.The results showed that the appropriate increase of sucrose without 2,4-D could slow down the occurrence of browning phenomenon and increase the bud differentiation rate.The highest doubling induction rate could be obtained on the medium with 40 g/L sucrose,15 g/L agar and 1.5 mg/L 6-BA.Two kinds of chimera plants were acquired,which were the one with both triploid ... |
| Keywords: | Welsh onion Callus Differential medium |
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