| 紫外分光光度法测定转基因烟草叶片中自藜芦醇的含量 |
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| 引用本文： | 毕琮,李臻,刘倩,刘炜.紫外分光光度法测定转基因烟草叶片中自藜芦醇的含量[J].山东农业科学,2011(1):93-96. |
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| 作者姓名： | 毕琮 李臻 刘倩 刘炜 |
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| 作者单位： | 1. 山东省农业科学院高新技术研究中心/山东省作物遗传改良与生物技术重点实验室,山东,济南,250100
2. 山东省农业科学院高新技术研究中心/山东省作物遗传改良与生物技术重点实验室,山东,济南,250100;农业部黄淮海作物遗传改良与生物技术重点开放实验室,山东,济南,250100 |
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| 基金项目： | 山东省农业科学院博士科研启动基金 |
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| 摘 要： | 利用紫外分光光度法对转基因烟草叶片中自藜芦醇的含量进行了测定。结果显示，转入花生白藜芦醇合成酶基因的烟草叶片中自藜芦醇浓度约为6．83μg/ml，比野生型材料中约5．11μg/ml的白藜芦醇含量略高。经紫外胁迫诱导后，其叶片中自藜芦醇含量分别提高到10．98μg/ml和9．25μg/ml。白藜芦醇合成酶转人烟草后不仅使目标植物中白藜芦醇含量有所增加，而且使植物自身对紫外胁迫的耐受能力也得到了较大的提高。
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| 关 键 词： | 紫外分光光度法 烟草 花生白藜芦醇合成酶 白藜芦醇 紫外胁迫 |
Determination of Resveratrol in Transgenic Tobacco Leaves by UV Spectrophotometry |
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| BI Cong,LI Zhen,LIU Qian,LIU Wei.Determination of Resveratrol in Transgenic Tobacco Leaves by UV Spectrophotometry[J].Shandong Agricultural Sciences,2011(1):93-96. |
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| Authors: | BI Cong LI Zhen LIU Qian LIU Wei |
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| Institution: | (1.High-tech Research Center,Shandong Academy of Agricultural Sciences/ Key Laboratory for Crop Genetic Improvement and Biotechnology of Shandong Province,Jinan 250100,China;2.Key Laboratory for Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture,Jinan 250100,China) |
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| Abstract: | The resveratrol content in transgenic tobacco leaves was determined by UV spectrophotometry.The results showed that the resveratrol concentration in transgenic tobacco leaves was about 6.83 μg/ml,which was slightly higher than that in wild type material(control) of 5.11 μg/ml.Under the UV stress,the resveratrol contents in transgenic material and control were up to 10.98 and 9.25 μg/ml respectively.The resveratrol synthetic enzyme gene could not only increase the resveratrol content,but also improve the tolerance of tobacco to UV stress. |
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| Keywords: | UV spectrophotometry Tobacco Peanut resveratrol synthetic enzyme Resveratrol UV stress |
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