Determination of residues of promecarb and its metabolite, 3-methyl-5-isopropylphenol,in cattle |
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| Authors: | Ellen M Burnett A E L Lanham |
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| Abstract: | A procedure is described for the colorimetric determination of promecarb as 3-methyl-5-isopropylphenol in tissues using 2,6-dichlorpbenzoquinone-4-chlorimine as the chromogen. Cattle were exposed to either 1, 3, 5 or 8 spray applications of a 0·2% promecarb emulsion at 3-day intervals or were fed promecarb at 2 ppm or 20 ppm daily in the diet for 20 days. All animals were slaughtered 30 h after final exposure. Of the animals in the spraying trial, residues in omental fat ranged from 0·9 ppm to 1·5 ppm promecarb and 0·4 ppm to 1·9 ppm 3-methyl-5-isopropylphenol. Residues in perirenal fat ranged from 0·8 ppm to 1·9 ppm promecarb and 0·9 ppm to 1·6 ppm 3-methyl-5-isopropylphenol. Residues in diaphragm muscle did not exceed 0·06 ppm. In the feeding trial, the maximum residues induced were 2·2 ppm promecarb and 1·4 ppm 3-methyl-5-isopropylphenol in omental fat of an animal fed for 20 days on a diet containing 20 ppm promecarb. |
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