| 南方镰孢Fusarium meridionale特异性PCR检测方法的建立与应用 |
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| 引用本文： | 王云飞,张 昊,杨美欣,雒丽丽,徐 进,许景升,冯 洁,陈万权.南方镰孢Fusarium meridionale特异性PCR检测方法的建立与应用[J].植物保护,2022,48(2):162-166. |
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| 作者姓名： | 王云飞 张 昊 杨美欣 雒丽丽 徐 进 许景升 冯 洁 陈万权 |
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| 作者单位： | 1. 甘肃农业大学植物保护学院, 兰州 730070; 2. 植物病虫害生物学国家重点实验室, 中国农业科学院植物保护研究所, 北京 100193; 3. 农业农村部国家植物保护甘谷观测实验站, 天水 741000 |
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| 基金项目： | 国家重点研发计划(2017YFE0126700, 2018YFD0200500); 国家现代农业小麦产业技术体系(CARS-03) |
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| 摘 要： | 为建立快速、稳定的南方镰孢Fusarium meridionale特异性检测方法，对已报道的镰孢属reductase-like基因部分序列进行比对分析，寻找特异性SNP位点，设计出特异性检测引物F-Fm/R-Fm3。利用该引物对包括南方镰孢在内的30株镰孢的基因组DNA进行PCR扩增。结果显示仅在7株南方镰孢中均扩增出400 bp左右的特异性条带。PCR灵敏度试验结果表明该方法的检测灵敏度达到500 pg基因组DNA。
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| 关 键 词： | 镰孢属 特异性检测 南方镰孢 |
| 收稿时间： | 2020/12/29 0:00:00 |
| 修稿时间： | 2021/1/7 0:00:00 |
Establishment and application of a specific PCR method for Fusarium meridionale detection |
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| WANG Yunfei,ZHANG Hao,YNAG Meixin,LUO Lili,XU Jin,XU Jingsheng,FENG Jie,CHEN Wanquan.Establishment and application of a specific PCR method for Fusarium meridionale detection[J].Plant Protection,2022,48(2):162-166. |
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| Authors: | WANG Yunfei ZHANG Hao YNAG Meixin LUO Lili XU Jin XU Jingsheng FENG Jie CHEN Wanquan |
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| Institution: | 1. College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3. National Agricultural Experimental Station for Plant Protection in Gangu, Ministry of Agriculture and Rural Affairs, Tianshui 741000, China |
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| Abstract: | In order to establish a rapid and stable PCR detection method for Fusarium meridionale, the partial reductase-like gene sequences from F.meridionale were analyzed by alignment to screen specific SNPs and a pair of specific primer (F-Fm/R-Fm3) were designed according to the specific SNPs. With the specific primers, the genomic DNAs of 30 strains from 21 Fusarium species, including Fusarium meridionale etc., were amplified by PCR. The results revealed that a specific amplicon of approximately 400 bp was amplified from seven strains of F. meridionale, while no amplicon was amplified from other 23 control strains and their negative control groups. Moreover, the sensitivity of this method reached 500 pg genomic DNA. |
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| Keywords: | Fusarium specific detection Fusarium meridionale |
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