| 钙离子响应蛋白GCaMP6s在稻瘟菌中的异源表达 |
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| 引用本文： | 林艾佳#,吴 奇#,倪瑞莲,陈 恒,卫 冕,李成云,王 一.钙离子响应蛋白GCaMP6s在稻瘟菌中的异源表达[J].植物保护,2022,48(2):9-15. |
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| 作者姓名： | 林艾佳# 吴 奇# 倪瑞莲 陈 恒 卫 冕 李成云 王 一 |
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| 作者单位： | 云南生物资源保护与利用国家重点实验室, 云南农业大学, 昆明 650201 |
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| 基金项目： | 国家重点研发计划(2017YFD0200400); 云南省基础研究计划青年基金(202101AU070101); 云南省教育厅科学研究基金(2020J0252); 省部共建云南生物资源利用与保护国家重点实验室科学研究基金(GZJS201903) |
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| 摘 要： | 稻瘟病严重威胁全球水稻的产量,钙离子信号通路参与了稻瘟菌生长、发育和致病,了解这些过程中菌株细胞质中钙离子浓度的变化对稻瘟病的防治具有重要意义.GCaMP6s作为一种细胞质钙离子感受蛋白在动物和植物中已有大量应用.本研究利用不同稻瘟菌菌株构建了 GCaMP6s基因的异源表达菌株,在0.01 g/mL钙离子处理下,GCa...
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| 关 键 词： | 稻瘟菌 异源表达 GCaMP6s |
| 收稿时间： | 2021/1/11 0:00:00 |
| 修稿时间： | 2021/2/1 0:00:00 |
Heterologous expression of Ca2+ sensor protein GCaMP6s in Magnaporthe oryzae |
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| LIN Aijia#,WU Qi#,NI Ruilian,CHEN Heng,WEI Mian,LI Chengyun,WANG Yi.Heterologous expression of Ca2+ sensor protein GCaMP6s in Magnaporthe oryzae[J].Plant Protection,2022,48(2):9-15. |
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| Authors: | LIN Aijia# WU Qi# NI Ruilian CHEN Heng WEI Mian LI Chengyun WANG Yi |
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| Institution: | State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | Rice blast caused by Magnaporthe oryzae is a serious threat to the global rice production. Calcium signaling pathway is involved in the growth, development and pathogenicity of M. oryzae. Understanding the changes of Ca2+ concentration in the cytoplasm of M. oryzae at three different stages is of great significance for control of rice blast. As a cytoplasmic Ca2+ sensor protein, GCaMP6s has been widely used in animals and plants. In this study, heterologous expression strains containing GCaMP6s were constructed using M. oryzae YN153 and YN157 strains. Under 0.01 g/mL Ca2+ treatment, GCaMP6s was stably expressed in the mycelia and conidia of M. oryzae. In addition, by comparing the colony diameter and sporulation between the heterologous expression strains of GCaMP6s and the wild type strains, we found that there was no difference between the two strains, indicating that the heterologous expression of GCaMP6s gene did not affect the mycelial growth and sporulation of M. oryzae. In this study, we obtained the heterologous expression of GCaMP6s in M. oryzae, which can provide research materials for further study on the changes of calcium ion concentration in the cytoplasm, and the growth, development and stress response of M. oryzae. |
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| Keywords: | Magnaporthe oryzae heterologous expression GCaMP6s |
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