| 鹅副粘病毒YG97分离株HN蛋白基因的克隆与序列分析 |
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| 引用本文： | 刘华雷,王永坤,朱国强,严维巍.鹅副粘病毒YG97分离株HN蛋白基因的克隆与序列分析[J].中国预防兽医学报,2002,24(5):334-337. |
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| 作者姓名： | 刘华雷 王永坤 朱国强 严维巍 |
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| 作者单位： | 1. 江苏省家禽科学研究所,江苏,扬州,225003
2. 扬州大学畜牧兽医学院,江苏,扬州,225009 |
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| 摘 要： | 鹅副粘病毒分离株YG97经10日龄鸡胚增殖后纯化，提取病毒的基因组RNA，采用RT－PCR一次性扩增出与预期设计的1.9kb大小相符合的特异性条带。将扩增产物提纯后克隆入pGEM^R-T载体，经转化、筛选及酶切鉴定后，初步获得了含鹅副粘病毒HN基因的阳性克隆，进一步进行了序列测定。序列分析表明所扩增的病毒的HN基因片段的长度为198bp，共编码571个氨基酸。同源性分析表明，YG97与LaSota毒株核苷酸同源性为79％，氨基酸的同源性为87％。与台湾1995年分离株Taiwan/95核苷酸和氨基酸的同源性分别为93％、96％，说明YG97与Taiwan/95亲缘关系较近，具有较高的相似性。与国内外部分发表的其它NDV毒株的核苷酸同源性在80％－84％之间，氨基酸的同源性在87％－91％之间。同源性分析表明YG97相对于经典的NDV在HN基因上发生了较大的变异。
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| 关 键 词： | 鹅 副粘病毒 YG97分离株 HN蛋白基因 克隆 序列分析 |
| 文章编号： | 1008-0589(2002)05-0334-04 |
| 修稿时间： | 2001年11月24 |
Cloning and Sequencing of HN Gene of Goose' s Paramyxovirus |
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| LIU Hua-lei ,WANG Yong-kun ,ZHU Guo-qiang ,YAN Wei-wei.Cloning and Sequencing of HN Gene of Goose'' s Paramyxovirus[J].Chinese Journal of Preventive Veterinary Medicine,2002,24(5):334-337. |
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| Authors: | LIU Hua-lei WANG Yong-kun ZHU Guo-qiang YAN Wei-wei |
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| Institution: | LIU Hua-lei 1,WANG Yong-kun 2,ZHU Guo-qiang 2,YAN Wei-wei 2 |
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| Abstract: | The genomic RNA of YG 97 were extracted.The hameagglutinin-neuraminidase (HN) gene of YG 97 has been successfully amplified RT-PCR and coloned in to pGEM-T vector.The sequence analysis showed that the nucleotide sequence of the HN gene was 1981 bp and encoding a protein of 571 amino acids.Sequence comparison between YG 97and the Taiwan/95 strains,the homology of the nucleotide was 93%,the homology of the amino acid was 96%,which indicated they probably had a common origin.The homology of the nucleotide compared with LaSota was 79%,the homology of the amino acid was 87%.The results indicated the mutation on the HN gene had varied largely,compared with classic NDH. |
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| Keywords: | Goose's Paramyxovirus Haemagglutinin-neuraminidase gene Sequenfce analysis homology |
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