Attenuation of Borrelia anserina by serial passage in liquid medium |
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| Authors: | J F Levine M J Dykstra W L Nicholson R L Walker G Massey H J Barnes |
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| Institution: | Department of Microbiology, Pathology and Parasitology, North Carolina State University, Raleigh 27606. |
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| Abstract: | Borrelia anserina (Sakharoff) was successfully grown in a liquid medium (Barbour-Stoenner-Kelly) for 39 passages. By the 12th serial passage in medium, infectivity of B anserina for chicks was lost. Electron microscopy did not reveal structural differences between non-infective and infective cultured organisms. Changes in the protein profiles were found by electrophoresis as the organisms were passed in culture. |
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