Identification of microsatellite markers for a leaf rust resistance gene introgressed into common wheat from Triticum timopheevii |
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| Authors: | I Leonova A Borner E Budashkina N Kalinina O Unger M Röuder E Salina |
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| Institution: | Institute of Cytology and Genetics, Russian Academy of Sciences, Lavrentieva ave. 10, Novosibirsk, 630090, Russia;Institut fur Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstr. 3, D-06466 Gatersleben;Nordsaat Saatzuchtge-sellschaft mbH, Hauptstrasse 1, D-38895 Bohnshausen, Germany |
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| Abstract: | The tetraploid wheat Triticum timopheevii Zhuk (AtAtGG) is known as a source of genes determining resistance to many diseases. An introgressive line 842, with durable resistance to leaf rust was established by crossing T. aestivum cv. ‘Saratovskaya29’ with T. timopheevii ssp. viticulosum and used for mapping leaf rust resistance genes. Molecular analysis of the line 842 with polymorphic microsatellite markers detected introgressions of T. timopheevii into the homoeologous group 2 chromosomes of common wheat. Transloca‐tion breakpoints of introgressed fragments were localized between the markers Xgwm95 and Xgwm817 on chromosome 2A, as well as Xgwm1128 and Xgwm1067 on chromosome 2B. Linkage analysis demonstrated the association of disease resistance at the seedling stage with chromosome 2A. The gene was found to be linked with marker Xgwm817 at a genetic distance of 1.5 cM. The alien leaf rust resistance gene was temporarily designated as lrTt1. |
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| Keywords: | Triticum timopheevii Puccinia recondita chromosome localization disease resistance introgressive lines microsatellite markers |
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