| 迟缓爱德华氏菌的粘附特性 |
| |
| 引用本文： | 欧阳志明,陈怀青.迟缓爱德华氏菌的粘附特性[J].南京农业大学学报,1997,20(3):87-91. |
| |
| 作者姓名： | 欧阳志明 陈怀青 |
| |
| 作者单位： | 南京农业大学动物医学院 |
| |
| 基金项目： | 江苏省九五重点攻关项目 |
| |
| 摘 要： | 用绵羊红细胞对不同来源的１５株迟缓爱德华氏菌作血凝试验。结果表明，１５株ＥＴ均具有血凝性，且不能为甘露糖，蔗糖，葡萄糖所抑制。分别用甲醛，戊二醛，胰蛋白酶处理ＥＴ的模式菌株ＥＴ９９菌体，能抑制细菌血凝。提纯的ＥＴ９９菌株的鞭毛蛋白，外膜蛋白均无血凝性。同时用ＨＥｐ－２细胞分析了１５株ＥＴ的细胞粘附特性。结果表明，有１０株ＥＴ能粘附ＨＥｐ－２细胞、粘附菌数大于１０ＣＦＵ／细胞。分别用甲醛，戊二醛，胰
|
| 关 键 词： | 兽医 迟缓爱德华氏菌 血凝性 细胞粘附 |
ADHESION PROPERTY OF EDWARDSIELLA TARDA |
| |
| Ouyang Zhiming,Chen Huaiqing and Lu Chengping.ADHESION PROPERTY OF EDWARDSIELLA TARDA[J].Journal of Nanjing Agricultural University,1997,20(3):87-91. |
| |
| Authors: | Ouyang Zhiming Chen Huaiqing and Lu Chengping |
| |
| Abstract: | Little information was available on the virulence mechanism of Edwardsiella tarda (ET). It was undetermined whether the bacterium initially adhered to its host and then established infection. The ability of ET adhesion was investigated in this paper. All of the tested 15 ET strains expressed mannose resistant hemagglutination (MRHA) against sheep erythrocytes. ET99 agglutinated erythrocytes which could not be inhibited by D mannose, sucrose or glucose. The hemagglutination of this bacterium were partially inhibited by trypsin, formaldehyde or glutaraldehyde. The adhesion of ET99 to HEp 2 cells were partially inhibited by pretreatment of ET99 or HEp 2 cell monolayers with trypsin, formaldehyde or glutaraldehyde. When ET99 was treated by hydrochloric acid, the amount of adhesive bacteria decreased. D mannose, sucrose or glucose could not inhibit ET99 adhesion, but purified flagellin of ET99 could do. It was suggested that the adhesin of ET was proteinaceous flagellum. |
| |
| Keywords: | Edwardsiella tarda cell adhesion hemagglutination |
| 本文献已被 CNKI 维普 等数据库收录！ |
