| 一株禾谷镰刀菌拮抗菌株的筛选及鉴定 |
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| 引用本文： | 胡元森,朱明杰,宇光海,王肖肖,李翠香,陈亮.一株禾谷镰刀菌拮抗菌株的筛选及鉴定[J].广西农业科学,2013(2):234-239. |
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| 作者姓名： | 胡元森 朱明杰 宇光海 王肖肖 李翠香 陈亮 |
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| 作者单位： | 河南工业大学生物工程学院,郑州450001 |
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| 基金项目： | 河南省教育厅自然科学基金项目（20108180008）;郑州市科技发展计划项目（2001NYXM224） |
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| 摘 要： | 【目的】筛选对禾谷镰刀菌具有高拈抗活性的微生物菌株，探索小麦赤霉病的生物防治方法。【方法】用稀释平板涂布法及平板对峙法从24份取白河南郑州近郊、信阳、新乡、商丘、开封等地区发生小麦赤霉病的出间上样中分离、筛选禾谷镰刀菌的拈抗菌株。【结果】从24份土样中分离纯化到65株菌株，对峙实验发现其中8株对禾谷镰刀菌具有拈抗作用，其中，菌株Jc-07的拮抗效果最佳。根据形态特征、生理生化特性和16SrDNA序列分析，将菌侏Jc-07初步鉴定为解淀粉芽孢杆菌（Bacillusamyloliquefaciens）。抑菌实验表明，菌株Jc-07发酵滤液明显抑制禾谷镰刀菌的菌丝生长，使禾谷镰刀菌孢子萌发率显著降低。【结论】筛选得到的菌株Jc-07对禾谷镰刀菌具有较高的拈抗活性，具有防治小麦赤霉病的潜在应用价值。
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| 关 键 词： | 禾谷镰刀菌 拈抗菌 筛选 解淀粉芽孢杆菌 |
Screening and identification of a bacterial strain with antagonistic activity to wheat Fusarium graminearum |
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| HU Yuan-sen,ZHU Ming-jie,YU Guang-hai,WANG Xiao-xiao,LI Cui-xiang,CHEN Liang.Screening and identification of a bacterial strain with antagonistic activity to wheat Fusarium graminearum[J].Guangxi Agricultural Sciences,2013(2):234-239. |
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| Authors: | HU Yuan-sen ZHU Ming-jie YU Guang-hai WANG Xiao-xiao LI Cui-xiang CHEN Liang |
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| Institution: | (Bioengineering College, Henan University of Technology, Zhengzhou 450001, China) |
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| Abstract: | Objective]The present studies were performed to isolate microbial strain with antagonistic activity to wheat Fusarium graminearum, and explore the biological control of wheat scab. Method ]The dilution spread plate method and the plate confrontation method were used to screen the antagonistic strains from 24 soil samples planted wheat with F. granffuearum disease previously. Result]There were 65 strains isolated from the samples and 8 stains were found to be antagonistic towards F. graminearum. Of the 8 isolates, strain Jc-07 showed the strongest inhibition effect. Based on the morphological, physiological and biochemical characteristics as well as the sequencing of 16S rDNA, the strain Jc-07 was preliminary identified as Bacillus amyloliquefaclens. Fungistatic activity assay result indicated that the cuhure filtrate of strain Jc-07 was effective in inhibiting the mycelium growth of F. graminearum,and significantly reduced the spore germi- nation rate. Conclusion]The strain Jc-07 displayed relatively high inhibition activity to Fusafum gramineumm amt had the potential in biological control of wheat scab. |
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| Keywords: | Fusarium graminearum fungistatic effect screening B amyloliquefaciens |
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