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不同砧木嫁接对西瓜抗生理性凋萎症的作用机理研究
引用本文:韦志扬,覃泽林,韦莉萍,韦昌联,麻小燕,温国泉,何毅.不同砧木嫁接对西瓜抗生理性凋萎症的作用机理研究[J].广西农业科学,2013(5):773-777.
作者姓名:韦志扬  覃泽林  韦莉萍  韦昌联  麻小燕  温国泉  何毅
作者单位:[1]广西农业科学院农业科技信息研究所,南宁530007 [2]广西农业科学院园艺研究所,南宁530007 [3]广西科学技术厅,南宁530012
基金项目:广西自然科学基金项目(2010GXNSFA013082)
摘    要:【目的】探明不同砧木嫁接对西瓜抗生理性凋萎症的作用机理,为抗性砧木筛选和育种提供理论依据。【方法】选用葫芦、南瓜、冬瓜、野生西瓜等12个砧木与广西三号无籽西瓜进行嫁接试验、田间栽培试验,测定不同嫁接组合对西瓜生长后期叶片生理生化指标的影响。【结果】不同的嫁接组合中,以白籽南瓜、广西本地葫芦、杂交葫芦、伙伴野生西瓜、日本南瓜、大蒲瓜、超丰F1作砧木西瓜生理性凋萎症发病率较低,为0~6.00%,最低的为白籽南瓜。发病率低的组合,其叶片叶绿素a、叶绿素b、总叶绿素含量和POD活性、SOD活性、Pro含量均极显著高于对照,MDA含量则显著低于对照。【结论】日本南瓜、广西本地葫芦、杂交葫芦、伙伴野生西瓜对西瓜生理性凋萎症抗性较强,可在生产上示范应用。

关 键 词:嫁接西瓜  生理性凋萎症  砧木筛选  POD  SOD  Pro  MDA

Effect mechanisms of different grafting rootstocks on physiological wilt disease of watermelon
WEI Zhi-yang,QIN Ze-lin,WEI Li-ping WEI Chang-lian,MA Xiao-yan,WEN Guo-quan,HE Yi.Effect mechanisms of different grafting rootstocks on physiological wilt disease of watermelon[J].Guangxi Agricultural Sciences,2013(5):773-777.
Authors:WEI Zhi-yang  QIN Ze-lin  WEI Li-ping WEI Chang-lian  MA Xiao-yan  WEN Guo-quan  HE Yi
Institution:1Agricultural Science and Technology Information Research Institute, Guangxi Academy of Agricuhural Sciences, Nanning 530007, China; 2Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3Guangxi Department of Science and Technology, Nanning 530012, China)
Abstract:Objective]The experiment was conducted to study the effect mechanisms of different rootstocks on physiologi- cal wilt disease of watermelon in order to provide theoretical foundation for resistant rootstock screening and watermelon breeding. Method]Twelve different rootstocks of Lagenaria siceran'a, squash, Benincasa and wild Citrullus vulgaris were se- lected to undergo the grafting test and filed cultivation test with seedless watermelon Guangxi 3. Physiological and biochemical indexes of watermelon leaves of different grafting combinations at later stage were determined. Result]The watermelon grafted with rootstocks of white seed pumpkin, Guangxi local eucurbit, cross cueurbit, Huoban wild watermelon, Japanese pumpkin, large white flowered gourd, and Chaofeng F1 generation showed low incidence rate of physiological wilt disease. The incidence rate was in 0-6.00%. The incidence rate of physiological wilt disease was the lowest when white seed pumpkin was used as root- stock. The chlorophyll a content, chlorophyll b content, total chlorophyll content, POD and SOD activity and free praline con- tent were all significantly higher than the control, while the MDA content was significantly lower than the control. Conclu- sion]Four rootstocks viz., white seed pumpkin, Guangxi local cueurbit, cross eueurbit, Huoban wild watermelon showed stronger resistance to physiological wilt disease, therefore they could be applied to watermelon production.
Keywords:gratting watermelon  physiological wilt disease  root stock selection  l~OIJ  30D  t'ro  MI)A
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