| 番茄溃疡病菌实时荧光PCR快速检测方法研究 |
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| 引用本文： | 吴兴海,邵秀玲,邓明俊,陈长法,厉艳,梁成珠.番茄溃疡病菌实时荧光PCR快速检测方法研究[J].江西农业学报,2007,19(3):34-36. |
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| 作者姓名： | 吴兴海 邵秀玲 邓明俊 陈长法 厉艳 梁成珠 |
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| 作者单位： | 山东出入境检验检疫局,山东,青岛,266002 |
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| 基金项目： | 国家质检总局植物病原检测专项基金 |
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| 摘 要： | 根据番茄溃疡病菌ITS(16S~23SrDNA间隔区)基因序列,设计并合成了荧光PCR检测引物(Fan1和Fan2)及特异性检测探针(Fanprobe),对番茄溃疡病菌和非番茄溃疡病菌的标准菌株实时荧光PCR反应。结果表明,番茄溃疡病菌PCR产物出现强烈的特异杂交信号,而非番茄溃疡病菌均未出现特异荧光信号,证明这对引物及探针具有番茄溃疡病菌鉴定特异性。将番茄溃疡病菌DNA做梯度稀释,测定该检测体系的敏感度,结果表明,此体系可检出52.3 fg/μL的模板。
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| 关 键 词： | 番茄溃疡病菌 实时荧光PCR 快速 检测方法 |
| 文章编号： | 1001-8581（2007）03-0034-03 |
| 收稿时间： | 2006-10-26 |
| 修稿时间： | 2006年10月26 |
Study on Rapid Detection of Clavibacter michiganensis subsp. michiganensis by Real-time Fluorescent PCR |
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| WU Xing-hai,SHAO Xiu-ling,DENG Ming-jun,CHEN Chang-fa,LI Yan,LIANG Cheng-zhu.Study on Rapid Detection of Clavibacter michiganensis subsp. michiganensis by Real-time Fluorescent PCR[J].Acta Agriculturae Jiangxi,2007,19(3):34-36. |
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| Authors: | WU Xing-hai SHAO Xiu-ling DENG Ming-jun CHEN Chang-fa LI Yan LIANG Cheng-zhu |
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| Institution: | Shandong Exit-entry Inspection and Quarantine Bureau, Qingdao 266002, China |
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| Abstract: | Specific primers and Taq Man probe,which were designed based on the sequence of ITS gene(16S-23SrDNA spacer region) in Clavibacter michiganensis subsp.michiganensis,were used to detect the standard bacteria from both Clavibacter michiganensis subsp.michiganensis and other germina by using real-time fluorescent PCR assay.The results showed that the PCR product from Clavibacter michiganensis subsp.michiganensis had strong specific fluorescent signal,while that from other germina hadn't,which indicated that the primers and Taq Man probe had the specificity to detect Clavibacter michiganensis subsp.michiganensis.The system could detect the DNA solution of 52.3fg/μL in the gradient dilution test of Clavibacter michiganensis subsp.michiganensis. |
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| Keywords: | Clavibacter michiganensis subsp michiganensis Real-time fluorescent PCR Rapid Detection |
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