| 山新杨组织培养快繁技术研究 |
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| 作者姓名： | 刘文萍 韩玉琴 南相日 朱财 |
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| 作者单位： | 黑龙江省农科院生物技术研究中心 哈尔滨150086
(刘文萍，韩玉琴，南相日)，黑龙江省农科院生物技术研究中心 哈尔滨150086(朱财) |
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| 基金项目： | 黑龙江省哈尔滨市科委计划项目(项目编号:2004AA6CN036)部分内容. |
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| 摘 要： | 山新杨冬季枝条经FC处理,选择萌发新枝的茎段和叶片为接种外植体;诱导茎段产生腋芽的培养基为1/2MS 6-BA0.2~0.5mg/L NAA0~0.5mg/L,诱导叶片产生不定芽的培养基为1/2MS或MS 6-BA0.5mg/L NAA0.5mg/L;丛生芽快繁培养基MS 6-BA0.3mg/L NAA0.3mg/L,每20天继代一次,繁殖倍数20左右;生根培养基为1/2MS或MS,附加0.1~0.5mg/L的多效唑,对生根有促进作用。
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| 关 键 词： | 山新杨 组织培养 快繁技术 |
Study on Rapid Multiplication Techniques by Tissue Culture in Shanxinyang(P.davidiana X P.bollena ) |
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| Authors: | Liu Wenping Han Yuqin Nan Xiangri Zhu Cai |
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| Abstract: | Stem segments and leaves of new shooting, which are from winter shoot treated by FC , was used as inoculating explant. The medium used for producing axillary bud from stem segment is 1/2MS 6-BA 0.2~0.5 mg/L NAA 0~0.5mg/L; the medium used for generating adventitious bud is 1/2MS or MS 6-BA 0.5mg/L NAA0.5mg/L; medium used for producing rosette buds is MS 6-BA0.3mg/L NAA0.3mg/L. Regeneration was conducted every 20 days and 20 times of multiplication could be achieved. Rooting medium is 1/2MS or MS. Adding benzimidazole 0.1~0.5mg/L in the medium could promote rooting. |
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| Keywords: | Shanxinyang(P davidiana X P bollena ) Tissue culture Rapid multiplication techniques |
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