| 辣椒离体再生体系研究 |
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| 作者姓名： | 黄 炜 巩振辉 |
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| 作者单位： | 西北农林科技大学园艺学院，西北农林科技大学园艺学院 陕西杨陵 712100，陕西杨陵 712100 |
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| 基金项目： | 国家自然科学基金（30571262）和陕西省农业分子生物学重点实验室项目（2002.2）. |
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| 摘 要： | 以5个辣椒(Capsicum annuum L．)品种(系)为材料,针对不同基因型、苗龄、外植体种类、激 素组合等因素对辣椒植株离体再生的影响进行了较为系统的研究,从“农城椒2号”、“农城椒3号”、 “0127”、“0159”、“0171”等5个辣椒品种(系)中筛选出“农城椒2号”、“0127”、“0171”3个再生能力 较强的基因型。确定了辣椒离体再生最适外植体为子叶,最适苗龄为12-14d。筛选出高效不定芽分 化培养基(MS 3％蔗糖 0．6％琼脂 5．0mg/L BA 0．5-1．0mg/L IAA),其不定芽诱导率可达98％。诱导 出的不定芽转入MS 3％蔗糖 0．6％琼脂 3．0mg/L BA 1．0mg/L IAA 2．0mg/L GA3 10．0mg/L AgNO3 芽伸长培养基,不定芽伸长率最高可达47．1％。不定芽伸长后在MS 3％蔗糖 0．6％琼脂 0．2mg/L I- AA 0．1mg/L NAA生根培养基上诱导不定根。待其长成发达根系后移栽大田成苗,建立了辣椒高效 植株再生体系。
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| 关 键 词： | 辣椒 植株再生 离体培养 |
Studies on the Plant in Vitro Regeneration System in Pepper |
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| Authors: | Huang Wei Gong Zhenhui |
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| Institution: | (College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100) |
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| Abstract: | This study established the program of a high efficient in vitro regeneration procedure of five pepper cultivars. The effect of genotypes, explants, hormones, and days of seedlings on the tissue culture in pepper was investigated systematically. Three cultivars of pepper (Capsicum annuum L.), ''Nongchengjiao No.2'', ''0127'', and ''0171'', were screened out of five cultivars for their high capacity of regeneration. The experimental results showed that the cotyledon explants taken from 12-day-old seedlings displayed the highest shoot-buds regeneration capacity. As far as culture medium was concerned, the buds induced well on MS 3% sucrose 0.6% agar 5.0mg/L BA 0.5-1.0mg/L IAA, which made the shoot buds differentiation frequency of 98%. Moreover, 45.7% of induced buds elongated well on the medium of MS 3% sucrose 0.6% agar 3.0mg/L BA 1.0mg/L IAA 2.0mg/L GA3 10.0mg/L AgNO3. The regenerated plants were transplanted into nutrient soil and developed into normal plants. So, an efficient program for plant regeneration from explants of pepper had been developed. |
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| Keywords: | Capsicum annuum L Plant regeneration In vitro culture |
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