| 表面活性剂SDS对草鱼急性毒性效应的研究 |
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| 作者姓名： | 孙翰昌 黄丽英 丁诗华 罗江兰 耿晓修 |
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| 作者单位： | 西南农业大学水产与水文学院水产生物技术实验室 重庆400716(孙翰昌，黄丽英，丁诗华，耿晓修)，上海师范大学生命与环境科学学院 上海200234(罗江兰) |
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| 基金项目： | 重庆市科委应用基础研究项目（N0.6869）. |
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| 摘 要： | 为了研究阴离子表面活性剂对鱼类的急性毒性效应,设计了不同浓度的十二烷基硫酸钠(SDS)溶液对草鱼进行暴露实验并测定其体内各组织SOD活性。急性毒性测试表明,SDS对草鱼的48h、96hLC50分别为11．8mg／L和5．2mg／L。低浓度的SDS(小于0．2mg/L)暴露对草鱼的超氧化物歧化酶(SOD)活性有极显著诱导作用,但随着SDS浓度升高和暴露时间延长,草鱼各组织SOD活性均呈明显的下降趋势,提示SDS暴露所引起的酶活性变化与暴露浓度和暴露时间有一定的相关性。这些结果显示,阴离子表面活性剂SDS对草鱼抗氧化酶有毒性效应,SOD活性变化可以作为评价环境中有毒污染物的生物敏感性指标。
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| 关 键 词： | 十二烷基硫酸钠 草鱼 超氧化物歧化酶 |
Acute Toxicological Effects of Sodium Dodecyl Sulfate on Ctenopharyngodon idellus |
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| Authors: | Sun Hanchang Huang Liying Ding Shihu Luo Jianglan Geng Xiaoxiu |
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| Institution: | (1Lab of Aquatic Biotechnology, College of Aquaculture and Hydrology, Southwest AgriculturalUniversity, Chongqing 400716; 2Life & Environment Science College Shanghai Normal University, Shanghai 200234) |
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| Abstract: | The activities of superoxide dismutase (SOD) in tissue of Ctenopharyngodon idellus were determined after 10 d-period exposure to different concentrations (0.01, 0.2 and 4.0 mg/L) of sodium dodecyl sulfate (SDS) in order to study the acute toxicological effects of anionic surfactants on fish. The results showed that the 48 h and 96 h LC50 of the acute toxicity tests of SDS to Ctenopharyngodon idellus were 11.8 mg/L and 5.2 mg/L. Induction of the activities of SOD in all tested tissues occurred in low concentrations (less than 0.2 mg/L), and then the enzyme activities decreased with increase of SDS concentration and extension of exposure time, suggesting that the enzyme activities correlated to the SDS concentration and the exposure time. All of the results concluded that there was a toxic effect of SDS on Ctenopharyngodon idellus and that SOD activities could be used as biomarkers to evaluate the deleterious pollutant in the environment. |
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| Keywords: | Sodium dodecyl sulfate Ctenopharyngodon idellus Superoxide dismutase |
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