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Development of tetraploid plants from an interspecific hybrid between mungbean (Vigna radiata) and rice bean (Vigna umbellata) |
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| Authors: | Tanapon Chaisan Prakit Somta Peerasak Srinives Sontichai Chanprame Rangsarid Kaveeta Surapong Dumrongkittikule |
| Institution: | 178. Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand 278. Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok, 10900, Thailand 378. Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand 478. Department of Agronomy, Faculty of Agriculture, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand 578. Department of Horticulture, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand |
| Abstract: | Mungbean (Vigna radiata) and rice bean (V. umbellata) (both species 2n = 2x = 22) have desirable traits that complement each other. In this study, we rescued embryos from a cross between mungbean cv. “Kamphaeng Saen 2” and rice bean cv. “Miyazaki” and resolved the hybrid sterility problem by colchicine treatment. The interspecific hybrids were obtained when Kamphaeng Saen 2 was used as the female parent. Four out of 80 immature seeds at 12 days old were able to germinate on an MS medium supplemented with 1 mg L?1 IAA, 0.2 mg L?1 kinetin, and 500 mg L?1 casein hydrolysate. Forty random amplified polymorphic DNA (RAPD) primers were screened for polymorphism among the parents, and two specific primers were finally chosen for testing of hybridity. Using the two primers, all putative F1 hybrids were confirmed as the interspecific hybrids. To observe their fertility, some of the hybrid seedlings were transplanted. The hybrid produced flowers profusely but failed to set pods. To overcome the sterility, plants were induced to become tetraploid by colchicine treatment in vitro. The ploidy level of the regenerated seedlings was confirmed from leaf DNA using a flow cytometer. Three out of 20 hybrid seedlings (15%) were successfully induced from diploid to tetraploid by a colchicine concentration of 2 g L?1. The tetraploid hybrids were able to produce flowers and set pods normally. |
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