| 黑龙江大豆轮作和连作土壤细菌群落多样性比较 |
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| 引用本文： | 朱英波,史凤玉,张瑞敬,武云鹏.黑龙江大豆轮作和连作土壤细菌群落多样性比较[J].植物保护学报,2014,41(4):403-409. |
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| 作者姓名： | 朱英波 史凤玉 张瑞敬 武云鹏 |
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| 作者单位： | 河北科技师范学院生命科技学院, 秦皇岛066600;河北科技师范学院生命科技学院, 秦皇岛066600;张家口市植保站, 河北 张家口075000;河北科技师范学院生命科技学院, 秦皇岛066600 |
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| 基金项目： | 河北省自然科学基金（C2014407021），中国博士后科学基金（20100480028），河北科技师范学院博士启动基金（2007YB010） |
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| 摘 要： | 为研究大豆轮作和连作对土壤细菌群落多样性的影响，提取黑龙江大豆轮作和连作土壤微生物总DNA，采用细菌通用引物27F和1492R扩增了土壤细菌16S rDNA片段，构建了细菌16S rDNA克隆文库，并对文库中的细菌群落多样性进行了分析。通过16S rDNA序列同源性分析，发现76.5%克隆序列与环境中未培养细菌的16S rDNA序列有较高的相似性，仅有23.5%的克隆序列与GenBank数据库中可培养细菌有较高的相似性，表明黑龙江大豆田土壤中的多数细菌尚未培养。系统发育分析表明黑龙江土壤细菌分属于6大类群，其中变形菌Proteobacteria和酸杆菌Acidobacteria所占比例较大，另有少量厚壁菌Firmicutes、放线菌Actinobacteria、芽单胞菌Gemmatimonadetes和未分类的细菌。大豆轮作土壤细菌多样性更为丰富，并以变形菌为优势细菌类群；而大豆连作土壤细菌多样性有所减少，以酸杆菌为优势细菌类群。表明黑龙江大豆田土壤中细菌多样性十分丰富，其种植方式对土壤细菌群落结构影响较大。
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| 关 键 词： | 大豆 轮作 连作 细菌多样性 16S rDNA克隆文库 |
| 收稿时间： | 2014/3/16 0:00:00 |
Comparison of bacterial diversity in rotational and continuous soybean cropping soils in Heilongjiang |
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| Zhu Yingbo,Shi Fengyu,Zhang Ruijing and Wu Yunpeng.Comparison of bacterial diversity in rotational and continuous soybean cropping soils in Heilongjiang[J].Acta Phytophylacica Sinica,2014,41(4):403-409. |
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| Authors: | Zhu Yingbo Shi Fengyu Zhang Ruijing and Wu Yunpeng |
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| Institution: | College of Life Sciences, Hebei Normal University of Science and Technology, Qinhuangdao 066600, Hebei Province, China;College of Life Sciences, Hebei Normal University of Science and Technology, Qinhuangdao 066600, Hebei Province, China;Zhangjiakou Plant Protection Station, Zhangjiakou 075000, Hebei Province, China;College of Life Sciences, Hebei Normal University of Science and Technology, Qinhuangdao 066600, Hebei Province, China |
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| Abstract: | Total DNA of the microbes in rotational and continuous soybean cropping soils in Heilongjiang were extracted and amplified using the bacterial general primers 27F and 1492R. The bacterial 16S rDNA clone libraries were constructed and analyzed to study the effects of soybean rotational and continuous cropping on the soil bacterial diversity. The 16S rDNA sequence homology analysis indicated that 76.5% clone sequences were similar to those of uncultured bacteria in the environment, while only 23.5% clone sequences were most closely related to those of cultured bacteria in GenBank, suggesting great potential for undeveloped bacterial resources available in soybean fields in Heilongjiang. Phylogenetic analysis also showed that the sequenced clones fell into 6 major lineages within the domain bacteria. Among them, members of the Proteobacteria and Acidobacteria were the dominant group. Firmicutes, Actinobacteria,Gemmatimonadetes and unclassified bacteria were less well represented. The bacterial diversity in rotational soils was rich, and the main bacterial group was Proteobacteria. But the bacterial diversity in soybean continuous cropping soils was somewhat decreased, and the main bacterial group was Acidobacteria. The results revealed high diversity of soil bacteria in soybean fields in Heilongjiang, and planting mode did have a significant impact on the soil bacterial community structure. |
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| Keywords: | soybean rotation continuous cropping bacterial diversity 16S rDNA clone library |
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