| 桃离体茎尖的超低温保存及植株再生 |
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| 引用本文： | 赵艳华,吴雅琴.桃离体茎尖的超低温保存及植株再生[J].园艺学报,2006,33(5):1042-1044. |
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| 作者姓名： | 赵艳华 吴雅琴 |
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| 作者单位： | (河北省农林科学院昌黎果树研究所, 河北昌黎066600) |
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| 基金项目： | 河北省农林科学院资助项目(A03-2-01-08) |
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| 摘 要： | 以简单玻璃化法为基本方法, 研究了影响桃离体茎尖超低温保存后存活率的因子———低温驯化时间、蔗糖预培养时间、玻璃化液处理时间及化冻后植株再生条件; 建立了较为适宜的超低温保存技术程序———选择继代培养30 d的试管材料, 5℃低温驯化3～4周, 在含017 mol/L蔗糖的固体培养基预培养2 d, 再经玻璃化液PVS3处理100 min后浸入液氮, 化冻后茎尖存活率可达60%以上。
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| 关 键 词： | 桃 离体茎尖 超低温保存 |
| 文章编号： | 0513-353X（2006）05-1042-03 |
| 收稿时间： | 2005-10-14 |
| 修稿时间： | 2005-10-142005-12-13 |
Cryopreservation of Shoot Tips from Peach and Its Regeneration |
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| Zhao Yanhua,Wu Yaqin.Cryopreservation of Shoot Tips from Peach and Its Regeneration[J].Acta Horticulturae Sinica,2006,33(5):1042-1044. |
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| Authors: | Zhao Yanhua Wu Yaqin |
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| Institution: | (Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Changli, Hebei 066600, China) |
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| Abstract: | The current paper studied factors that effected the cryop reservation of peach in vitro shoot tips. By using simple vitrification technique, factors like, cold hardening, sucrose p reculture and PVS3 treatment time and regeneration condition were all tested and a suitable p rocedure was established at last. The results showed that when shoot tip swere excised from healthy in vitro plants of peach cultivars, which had been subcultured for 30 days and followed by 5℃ cold hardening for 3 - 4 weeks, precultured with 0.7 mol/L sucrose for two days, and dehydrated with PVS3 for 100 min before direct plunging into liquid nitrogen, the survival after cryop reservation was higher than 60%. |
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| Keywords: | Peach Shoot tip Cryopreservation |
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