| 发根农杆菌介导的向日葵遗传转化 |
| |
| 引用本文： | 陶均,谭汝芳,李玲.发根农杆菌介导的向日葵遗传转化[J].作物学报,2006,32(5):743-748. |
| |
| 作者姓名： | 陶均 谭汝芳 李玲 |
| |
| 作者单位： | 华南师范大学生命科学学院，广东省植物发育生物工程重点实验室，广东广州510631 |
| |
| 摘 要： | 本研究首次报道了发根农杆菌介导的向日葵遗传转化和毛状根形成的优化条件。由发根农杆菌诱导向日葵子叶和下胚轴形成的根在含卡那霉素（200 mg/L，kanamycin）的选择培养基(1/2 MS)上选择，12 d后存活的根转到无激素培养基上能快速生长，经分子检测证实其为毛状根。同时着重研究了不同诱导条件对向日葵毛状根形成的影响。结果发现适用于毛状根形成的最优条件为菌株R1205，菌液浓度（OD600）1.0，15 min感染，共培养3 d，培养基pH 6.5，30 μmol/L乙酰丁香酮（acetosyingone）。在此条件下，子叶和下胚轴的毛状根形成率分别达到23.7％和81.6％。
|
| 关 键 词： | 向日葵 发根农杆菌 毛状根 优化条件 |
| 收稿时间： | 2005-04-15 |
| 修稿时间： | 2005-10-06 |
Genetic Transformation of Sunflower (Helianthus Annuus L. ) Mediated by Agrobacterium rhizogenes |
| |
| TAO Jun,TAN Ru-Fang,LI Ling.Genetic Transformation of Sunflower (Helianthus Annuus L. ) Mediated by Agrobacterium rhizogenes[J].Acta Agronomica Sinica,2006,32(5):743-748. |
| |
| Authors: | TAO Jun TAN Ru-Fang LI Ling |
| |
| Institution: | Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631，Guangdong |
| |
| Abstract: | The transformation of Helianthus annuus L. mediated by Agrobacterium rhizogenes was reported. Almost all roots induced by different Agrobacterium rhizogenes bacterial strains which could survive in the selection medium (1/2 MS)containing 200 mg/L kanamycin were putative hairy roots. The effects of bacterial strains, bacterial concentrations,acetosyingone, silver nitrate and co-cultivation pH on sunflower transformation were investigated. For sunflower transformation, the optimal conditions were strain R1205, 1.0 bacterial concentration ( OD600 ), 15 minutes- infection, cocultivation for 3 days as well as 30 μmol/L acetosyingone (without silver nitrate) and pH 6.5. Under this condition, the hairy root production frequency was 23.7 % (cotyledon) or 81.6 % ( hypocotyl). |
| |
| Keywords: | Sunflower Agrobacterium rhizogenes Hairy root Optimal treatment condition |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《作物学报》浏览原始摘要信息 |
| 点击此处可从《作物学报》下载免费的PDF全文 |
