| Adventitious shoot regeneration of Platanus acerifolia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation |
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| 作者单位： | Li Zhi-neng Liu Guo-feng Fang Fang Bao Man-zhu* Key Laboratory of Horticultural Plant Biology，Ministry of Education，College of Horticulture and Forestry Sciences，Huazhong Ag- ricultural University，Wuhan 430070，P. R. China |
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| 基金项目： | 国家自然科学基金;武汉科技局资助项目 |
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| 摘 要： | The effects of Timentin and cefotaxime (Cef) on shoot regeneration of the London plane tree (Platanus acerifolia Willd.) and their use for the suppression of Agrobacterium tumefaciens in Agrobacterium-mediated genetic transformation were compared. Shoot regeneration was significantly reduced on the media with Cef at concentrations from 100 to 500 mg·L–1. Timentin showed negative effect on plant regeneration at concentrations of 100 and 500 mg·L–1; however, 300 mg·L–1 Timentin was shown to facilitate shoot r...
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| 关 键 词： | 悬铃木 根癌农杆菌 基因转化 抗生素 特美汀 抑制 不定芽 植株再生 |
| 收稿时间： | 2006-06-26 |
| 修稿时间： | 2006-10-10 |
Adventitious shoot regeneration of <Emphasis Type="Italic">Platanus acerifolia</Emphasis> Willd. facilitated by Timentin,an antibiotic for suppression of <Emphasis Type="Italic">Agrobacterium tumefaciens</Emphasis> in genetic transformation |
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| Authors: | Li Zhi-neng Liu Guo-feng Fang Fang Bao Man-zhu |
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| Institution: | Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, P. R. China |
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| Abstract: | The effects of Timentin and cefotaxime (Cef) on shoot regeneration of the London plane tree (Platanus acerifolia Willd.) and their use for the suppression of Agrobacterium tumefaciens in Agrobacterium-mediated genetic transformation were compared. Shoot regeneration was significantly reduced on the media with Cef at concentrations
from 100 to 500 mg·L−1. Timentin showed negative effect on plant regeneration at concentrations of 100 and 500 mg·L−1; however, 300 mg·L−1 Timentin was shown to facilitate shoot regeneration significantly and the regeneration frequency increased from 64% (control)
to 88%. Effective suppression of A. tumefaciens could be obtained with 500 mg·L−1 Cef, but plant regeneration was completely inhibited at this level. The A. tumefaciens on infected P. acerifolia leaf tissues was visually undetectable after three subcultures on a medium with 300 mg·L−1 Timentin. Considering the effect of Cef and Timentin on plant regeneration and suppression of Agrobacteria, Timentin at 300
mg·L−1 is the preferred application in A. tumefaciens-mediated transformation of P. acerifolia. |
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| Keywords: | Platanus acerifolia Agrobacterium tumefaciens leaf explant plant regeneration genetic transformation antibiotic Timentin |
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