| 布鲁氏菌内蒙古分离株omp31基因的克隆与序列分析 |
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| 引用本文： | 陆静,关平原,乌日罕,特木尔巴根,马立峰,菊花.布鲁氏菌内蒙古分离株omp31基因的克隆与序列分析[J].畜牧与饲料科学,2009,30(10):15-17. |
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| 作者姓名： | 陆静 关平原 乌日罕 特木尔巴根 马立峰 菊花 |
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| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018
2. 内蒙古动物疫病预防与控制中心,内蒙古,呼和浩特,010010 |
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| 摘 要： | 对三株布鲁氏菌内蒙古分离株的omp31基因进行克隆与序列分析.参照GenBank中布鲁氏菌的omp31基因序列,设计一对特异性引物,用PCR方法扩增布鲁氏菌omp31基因,对PCR产物进行克隆、测序,将测定序列拼接后与GenBank中获得的参考毒株omp31基因序列进行了比较.结果显示,三株布鲁氏菌分离株的omp31基因开放阅读框核苷酸序列同源性均为100.0%;与Brucella ceti B14/94、Brucella neotomae 5K33、Brucella suis 1330三个参考毒株omp31基因核苷酸序列同源性最高,为100.0%;与Brucella canis RM6/66、Brucella melitensis 183、Brucella melitensis 16M三个参考毒株omp31基因核苷酸序列同源性为99.9%;与Brucella melitensis Rev1参考毒株omp31基因核苷酸序列同源性为99.6%;与Brucella ovis ATCC 25840参考毒株omp31基因核苷酸序列同源性最低,为98.8%.
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| 关 键 词： | 布鲁氏菌 序列分析 |
Cloning and Sequence Analysis of omp31 Genes of Brucella Strain Isolated from Inner Mongolia |
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| LU Jing,GUAN Ping-yuan,Wurihan,Temuerbagen,MA Li-feng,Juhua.Cloning and Sequence Analysis of omp31 Genes of Brucella Strain Isolated from Inner Mongolia[J].Animal Husbandry and Feed Science,2009,30(10):15-17. |
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| Authors: | LU Jing GUAN Ping-yuan Wurihan Temuerbagen MA Li-feng Juhua |
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| Institution: | LU Jing1,GUAN Ping-yuan1,Wurihan2,Temuerbagen2,MA Li-feng2,Juhua2 (1.College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China,2.Central of Animal Disease Prevention and Control of Inner Mongolia,Hohhot 010010,China) |
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| Abstract: | Sequence analysis was conducted on genes of omp31 from 3 different Brucella strain isolated from Inner Mongolia.A pair of primers were designed for amplification of Brucella omp31 genes according to the reported of Brucella omp31 genes complete sequence.omp31 genes were amplified from 3 different Brucella strains by polymerase chain reaction (PCR),and the PCR products were cloned into pMD19-T vector and sequences were analyzed.The results showed nucleotide sequences in homology among 3 different Brucella is... |
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| Keywords: | omp31 |
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