Direct somatic embryogenesis and plant regeneration from leaf cultures of ornamental species of Dianthus |
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| Institution: | 1. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China;2. College of Biology and the Environment, Nanjing Forestry University, Nanjing, Jiangsu 210037, China;3. Department of Botany, Smithsonian Institution, Washington, DC 20013, USA;4. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;5. Environmental Futures Research Institute, Griffith University, Nathan, Brisbane 4111, Australia;6. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210046, China;7. Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA |
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| Abstract: | Protocol for direct somatic embryogenesis from leaf explants of economically important species of Dianthus, viz. D. caryophyllus, D. barbatus and D. chinensis has been developed. Murashige and Skoog’s (MS) liquid medium supplemented with 2,4-D (1 mg/l) was used for direct induction of somatic embryogenesis without an intervening callus phase. Initially globular structures were observed after 21 days of culture of leaf explants in liquid medium. Development of embryos to heart and torpedo stages was achieved in the liquid medium incorporated with polyethylene glycol (PEG 6000) at a concentration of 2.5%. Embryo maturation was further promoted by addition of casein hydrolysate (CH) (200 mg/l) in MS liquid medium. Embryos germinated to form plantlets on solid MS medium supplemented with GA3 (1 mg/l). Regenerated plants with well-developed root and shoot systems were successfully transferred to field conditions. |
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