| 离子色谱法测定乳制品中亚硝酸盐和硝酸盐含量的方法改进 |
| |
| 引用本文： | 武伦玮,胡雪,段国霞,唐烁,陈静,刘春霞,宫慧丽,任玉菊,刘丽君,李翠枝.离子色谱法测定乳制品中亚硝酸盐和硝酸盐含量的方法改进[J].乳业科学与技术,2020,43(6):20-25. |
| |
| 作者姓名： | 武伦玮 胡雪 段国霞 唐烁 陈静 刘春霞 宫慧丽 任玉菊 刘丽君 李翠枝 |
| |
| 作者单位： | 内蒙古伊利实业集团股份有限公司, 内蒙古呼和浩特 010110 |
| |
| 基金项目： | “十三五”国家重点研发计划重点专项（2017YFE0110800） |
| |
| 摘 要： | 改进GB 5009.33—2016《食品安全国家标准 食品中亚硝酸盐和硝酸盐的测定》中离子色谱法测定乳制品（乳粉、生牛乳）中亚硝酸盐和硝酸盐的方法。乳制品中亚硝酸盐和硝酸盐经超纯水提取后，加入乙酸溶液沉淀蛋白，AS11-HC色谱柱分离，电导检测器检测。结果表明：在本研究条件下，亚硝酸盐和硝酸盐的标准曲线均线性关系良好，相关系数均大于0.99；纯牛乳中亚硝酸盐的定量限为0.25 mg/kg、硝酸盐的定量限为0.8 mg/kg，乳粉中亚硝酸盐的定量限为1 mg/kg、硝酸盐的定量限为5 mg/kg；乳粉和纯牛乳中亚硝酸盐、硝酸盐回收率均能达到80%～110%，方法操作便捷、成本低、回收率理想、精密度较高。该法优化了国标检测方法的条件，解决了其中可能遇到的问题，可以更加快速、准确地检测乳制品中低含量亚硝酸盐和硝酸盐。
|
| 关 键 词： | 亚硝酸盐 硝酸盐 离子色谱法 乳制品 电导检测器 |
An Improved Ion Chromatography Method for Determination of Nitrite and Nitrate Contents in Milk and Milk Products |
| |
| WU Lunwei,HU Xue,DUAN Guoxia,TANG Shuo,CHEN Jing,LIU Chunxia,GONG Huili,REN Yuju,LIU Lijun,LI Cuizhi.An Improved Ion Chromatography Method for Determination of Nitrite and Nitrate Contents in Milk and Milk Products[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2020,43(6):20-25. |
| |
| Authors: | WU Lunwei HU Xue DUAN Guoxia TANG Shuo CHEN Jing LIU Chunxia GONG Huili REN Yuju LIU Lijun LI Cuizhi |
| |
| Institution: | (Inner Mongolia Yili Industrial Group Co. Ltd., Hohhot 010110, China) |
| |
| Abstract: | In order to develop a new method to determine nitrite and nitrate in milk and milk products, some improvements
were made on the ion chromatography method specified in the national standard GB 5009.33-2016 Determination of Nitrite
and Nitrate in Food. Samples were extracted with ultra-pure water and added with acetic acid to precipitate proteins before
being chromatographed on AS11-HC column and detected with a conductivity detector. Under the above conditions, the
calibration curves for nitrite and nitrate were linear with correlation coefficients greater than 0.99. The limit of quantitation
(LOQ) for nitrite and nitrate was 0.25 and 0.8 mg/kg in milk, and 1 and 5 mg/kg in milk powder, respectively. The recoveries
for spiked samples of milk powder and milk were from 80% to 110%. The improved method was simple to operate and
cheap with ideal recovery and high precision. It could avoid potential problems encountered in the use of the traditional
method and allow for more rapid and accurate detection of low contents of nitrite and nitrate in milk and milk products. |
| |
| Keywords: | nitrite nitrate ion chromatography dairy products conductivity detector |
|
| 点击此处可从《乳业科学与技术》浏览原始摘要信息 |
| 点击此处可从《乳业科学与技术》下载免费的PDF全文 |
